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Theses

Développement de l'imagerie de perfusion cérébrale par marquage des spins artériels

Abstract : This PhD work, mainly methodological, focused on the techniques of magnetic resonance imaging (MRI) to measure cerebral blood flow (CBF) and more particularly on arterial spin labeling (ASL), which uses water protons from the blood as markers. We implemented the pseudo-continuous ASL labeling sequence (pCASL) and evaluated its response to a hypercapnic stimulus. We evaluated different strategies to maximize the labeling inversion effeciency. For this, we implemented numerical simulation tools of ASL approaches. Our results demonstrated that the inversion efficiency is influenced by the homogeneity of the magnetic field in the labeling region, which becomes a problem at high magnetic field. The optimized pCASL protocol was then evaluated in rats at three magnetic fields (4.7, 7, and 11.7T) and compared with a conventional continuous ASL approach (CASL). This comparison showed excellent inter-animal and inter-field reproducibility of the developed method. In a second part, we evaluated the influence of the longitudinal relaxation time (T1) of brain tissue on the final CBF values. For this, we modified the tissue T1 by an intracerebral injection of manganese. This study demonstrated the difficulty of taking into account the change in T1 of the tissue. In a third part, we evaluated the contribution of a specific labeling coil for the CASL approach by comparing measurements of CBF obtained with that of pCASL. We found a good agreement between these two methods for all slices. Our results also illustrate the significant contribution of magnetization transfer effects in CASL sequence. The tools developed during this thesis were also used in several preclinical studies including brain tumors, stroke and head trauma models.
Keywords : MRI Brain
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Clément Debacker. Développement de l'imagerie de perfusion cérébrale par marquage des spins artériels. Neurosciences [q-bio.NC]. Université de Grenoble, 2014. Français. ⟨NNT : 2014GRENY018⟩. ⟨tel-01558846⟩

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