Mécanismes cellulaires et moléculaires potentiellement impliqués dans la régulation du stock de cellules souches spermatogoniales chez le poisson-zèbre Danio rerio

Abstract : By their ability to self-renew or differentiate into gametes, the spermatogonial stem cells (SSCs) are essential for sperm production throughout the life of male. The work done during the thesis had for objective the understanding of the cellular and molecular mechanisms potentially involved in the fate of the SSC in the teleost fish. With a new line of transgenic zebrafish expressing GFP under the control of a new gene promoter fragment vasa, we characterized a subpopulation of undifferentiated spermatogonia composed of asymmetric doublet. The observation of the GFP protein indicates that these doublets can fragment to generate isolated spermatogonia. Transplantation of fluorescent spermatogonial cells into the abdominal cavity of a recipient embryo shows that these cells have all functional properties of SSC. All our observations suggest that asymmetric doublet constitute a pool of stem cells with bivalent fate:they can continue their sperm differentiation or participate in the dynamic renewal of SSC pool. A rnaseq based transcritpome analysis of transcripts expressed in undifferentiated spermatogonia fluorescent and Sertoli cells highlights the diversity of paracrine regulatory pathways potentially involved in the control of the SSC (cell adhesion molecules, cytokines, growth factors, primary metabolites). Phylogenetic analyzes show that some of these regulatory pathways are well conserved among all Gnathostomes while others were lost in tetrapods or mammals only. A comparative approach initiated in rainbow trout generic confirms the interest of the data acquired in th
Document type :
Theses
Complete list of metadatas

https://tel.archives-ouvertes.fr/tel-01538419
Contributor : Abes Star <>
Submitted on : Tuesday, June 13, 2017 - 3:26:07 PM
Last modification on : Thursday, February 28, 2019 - 1:32:30 AM
Long-term archiving on : Tuesday, December 12, 2017 - 2:14:36 PM

File

fix_Kn9kdz8Dcurran.pdf
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-01538419, version 1

Collections

Citation

Edouard Curran. Mécanismes cellulaires et moléculaires potentiellement impliqués dans la régulation du stock de cellules souches spermatogoniales chez le poisson-zèbre Danio rerio. Agronomie. Agrocampus Ouest, 2016. Français. ⟨NNT : 2016NSARH099⟩. ⟨tel-01538419⟩

Share

Metrics

Record views

256

Files downloads

89