The molecular architecture of the nuclear pore complex, Nature, vol.33, issue.7170, pp.450695-701, 2007. ,
DOI : 10.1038/nature06405
Natural Evolution of Broadly Neutralizing Antibodies, Cell, vol.161, issue.3, pp.427-428, 2015. ,
DOI : 10.1016/j.cell.2015.04.007
URL : http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4426205
Basis of Cargo Recognition by Importin ??s: The Power of Structure, Structure, vol.23, issue.2, pp.251-252, 1993. ,
DOI : 10.1016/j.str.2015.01.005
HEAT repeats in the Huntington's disease protein, Nature Genetics, vol.101, issue.2, pp.115-116, 1995. ,
DOI : 10.1083/jcb.129.2.321
Long-Acting Integrase Inhibitor Protects Macaques from Intrarectal Simian/Human Immunodeficiency Virus, Science, vol.200, issue.6, pp.3431151-1154, 2014. ,
DOI : 10.1084/jem.20041196
URL : http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4308974
A long-acting integrase inhibitor protects female macaques from repeated high-dose intravaginal SHIV challenge, Science Translational Medicine, vol.27, issue.5415, pp.270-274, 2015. ,
DOI : 10.1126/science.284.5415.816
URL : http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4449736
Importin ??3 Interacts with HIV-1 Integrase and Contributes to HIV-1 Nuclear Import and Replication, Journal of Virology, vol.84, issue.17, pp.8650-8663, 2010. ,
DOI : 10.1128/JVI.00508-10
URL : http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2919037
Multiple Importins Function as Nuclear Transport Receptors for the Rev Protein of Human Immunodeficiency Virus Type 1, Journal of Biological Chemistry, vol.281, issue.30, pp.28120883-20890, 2006. ,
DOI : 10.1074/jbc.M602189200
Elite controllers as a model of functional cure, Current Opinion in HIV and AIDS, vol.6, issue.3, pp.181-187, 2011. ,
DOI : 10.1097/COH.0b013e328345a328
controlled intervention trial of male circumcision for reduction of HIV infection risk : the ANRS 1265 Trial, PLoS medicine, vol.2, issue.11, p.298 ,
The role of Ran in nuclear function, Current Opinion in Cell Biology, vol.12, issue.3, pp.302-307, 2000. ,
DOI : 10.1016/S0955-0674(00)00093-4
Core histones and linker histones are imported into the nucleus by different pathways, European Journal of Cell Biology, vol.80, issue.11, pp.80669-677, 2001. ,
DOI : 10.1078/0171-9335-00208
Normal Mode Analysis of Biomolecular Structures: Functional Mechanisms of Membrane Proteins, Chemical Reviews, vol.110, issue.3, pp.1463-1497, 2010. ,
DOI : 10.1021/cr900095e
Past, present and future: 30 years of HIV research, Nature Reviews Microbiology, vol.27, issue.12, pp.11877-883, 2013. ,
DOI : 10.1038/nrmicro3132
alpha Helix-RNA Major Groove Recognition in an HIV-1 Rev Peptide-RRE RNA Complex, Science, vol.273, issue.5281, pp.2731547-1551, 1996. ,
DOI : 10.1126/science.273.5281.1547
HIV-1 Latency: An Update of Molecular Mechanisms and Therapeutic Strategies, Viruses, vol.2, issue.4, pp.1715-1758, 2014. ,
DOI : 10.1002/bies.201200170
The Requirement of H1 Histones for a Heterodimeric Nuclear Import Receptor, Journal of Biological Chemistry, vol.277, issue.36, pp.32480-32489, 2002. ,
DOI : 10.1074/jbc.M202765200
Crystallization and Initial X-Ray Diffraction Characterization of Complexes of FxFG Nucleoporin Repeats with Nuclear Transport Factors, Journal of Structural Biology, vol.131, issue.3, pp.240-247, 2000. ,
DOI : 10.1006/jsbi.2000.4297
Structural basis for the interaction between NTF2 and nucleoporin FxFG repeats, The EMBO Journal, vol.21, issue.12, pp.2843-2853, 2002. ,
DOI : 10.1093/emboj/cdf305
Structural Basis for the Interaction between FxFG Nucleoporin Repeats and Importin-?? in Nuclear Trafficking, Cell, vol.102, issue.1, pp.99-108, 2000. ,
DOI : 10.1016/S0092-8674(00)00014-3
GLFG and FxFG Nucleoporins Bind to Overlapping Sites on Importin-beta, Journal of Biological Chemistry, vol.277, issue.52, pp.27750597-50606, 2002. ,
DOI : 10.1074/jbc.M209037200
Nanoscale stiffness topography reveals structure and mechanics of the transport barrier in intact nuclear pore complexes, Nature Nanotechnology, vol.4, issue.1, pp.60-64, 2015. ,
DOI : 10.1038/nnano.2014.262
Conformational selection in the recognition of the snurportin importin beta binding domain by importin beta, Biochemistry, issue.24, pp.495042-5047, 2010. ,
RanBP1 is crucial for the release of RanGTP from importin ??-related nuclear transport factors, FEBS Letters, vol.270, issue.2-3, pp.249-254, 1997. ,
DOI : 10.1016/S0014-5793(97)01467-1
RanGAP1 induces GTPase activity of nuclear Ras-related Ran., Proceedings of the National Academy of Sciences, vol.91, issue.7, pp.912587-2591, 1994. ,
DOI : 10.1073/pnas.91.7.2587
URL : http://www.ncbi.nlm.nih.gov/pmc/articles/PMC43414
Co-activation of RanGTPase and inhibition of GTP dissociation by Ran-GTP binding protein RanBP1, The EMBO journal, vol.14, issue.4, pp.705-715, 1995. ,
Catalysis of guanine nucleotide exchange on Ran by the mitotic
regulator RCC1, Nature, vol.354, issue.6348, pp.35480-82, 1991. ,
DOI : 10.1038/354080a0
The emerging role of native mass spectrometry in characterizing the structure and dynamics of macromolecular complexes, 2015. ,
URL : https://hal.archives-ouvertes.fr/hal-01164101
Influenza A Virus Polymerase: Structural Insights into Replication and Host Adaptation Mechanisms, Journal of Biological Chemistry, vol.285, issue.37, pp.28528411-28417, 2010. ,
DOI : 10.1074/jbc.R110.117531
URL : http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2937865
Optimization of protein purification and characterization using Thermofluor screens, Protein Expression and Purification, vol.91, issue.2, pp.192-206, 2013. ,
DOI : 10.1016/j.pep.2013.08.002
The development of antiretroviral therapy and its impact on the HIV-1/AIDS pandemic, Antiviral Research, vol.85, issue.1, pp.1-18, 2010. ,
DOI : 10.1016/j.antiviral.2009.10.002
Structural Evidence for Common Ancestry of the Nuclear Pore Complex and Vesicle Coats, Science, vol.22, issue.7, pp.3221369-1373, 2008. ,
DOI : 10.1016/j.tig.2006.05.007
Gastrointestinal tract and the mucosal macrophage reservoir in HIV infection. Clinical and vaccine immunology : CVI, pp.1469-1473, 2014. ,
Loading and Unloading: Orchestrating Centrosome Duplication and Spindle Assembly by Ran/Crm1, Cell Cycle, vol.4, issue.11, pp.41510-1514, 2005. ,
DOI : 10.4161/cc.4.11.2187
URL : http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1402358
Integrated Structural Analysis of the Human Nuclear Pore Complex Scaffold, Cell, vol.155, issue.6, pp.1551233-1243, 2013. ,
DOI : 10.1016/j.cell.2013.10.055
Generation of GTP-bound Ran by RCC1 is required for chromatin-induced mitotic spindle formation, Nature, issue.6740, pp.400178-181, 1999. ,
Health-Related Quality of Life After 1 Year of Highly Active Antiretroviral Therapy, JAIDS Journal of Acquired Immune Deficiency Syndromes, vol.32, issue.1, pp.38-47, 1999. ,
DOI : 10.1097/00126334-200301010-00006
The arginine-rich RNA-binding motif of HIV-1, 2013. ,
Therapeutic Targeting of Nuclear Protein Import in Pathological Cell Conditions, Pharmacological Reviews, vol.61, issue.3, pp.61358-372, 2009. ,
DOI : 10.1124/pr.108.000620
Applying mass spectrometry to study non-covalent biomolecule complexes, Mass Spectrometry Reviews, vol.84, issue.1, 2015. ,
DOI : 10.1002/mas.21462
Structural basis for the selective nuclear import of the C2H2 zinc-finger protein Snail by importin ??, Acta Crystallographica Section D Biological Crystallography, vol.6, issue.4, pp.701050-1060, 2014. ,
DOI : 10.1107/S1399004714000972/yt5062sup1.pdf
Identification and Characterization of a General Nuclear Translocation Signal in Signaling Proteins, Molecular Cell, vol.31, issue.6, pp.31850-861, 2008. ,
DOI : 10.1016/j.molcel.2008.08.007
Molecular Basis for the Recognition of a Nonclassical Nuclear Localization Signal by Importin ??, Molecular Cell, vol.10, issue.6, pp.1345-1353, 2002. ,
DOI : 10.1016/S1097-2765(02)00727-X
Structure of importin-beta bound to the IBB domain of importin-alpha, Nature, issue.6733, pp.399221-229, 1999. ,
Controlling HIV-1 Rev Function, Current Drug Targets - Immune, Endocrine & Metabolic Disorders, vol.4, issue.4, pp.287-295, 2004. ,
DOI : 10.2174/1568008043339677
Identification of sequences important in the nucleolar localization of human immunodeficiency virus Rev : relevance of nucleolar localization to function, Journal of Virology, vol.64, issue.2, pp.881-885, 1990. ,
Prevention of HIV-1 Infection with Early Antiretroviral Therapy, New England Journal of Medicine, vol.365, issue.6, pp.493-505, 2011. ,
DOI : 10.1056/NEJMoa1105243
Reduction of maternal-infant transmission of human immunodeficiency virus type 1 with zidovudine treatment. Pediatric AIDS Clinical Trials Group Protocol 076 Study Group, pp.3311173-1180, 1994. ,
Crystallographic analysis of the recognition of a nuclear localization signal by the nuclear import factor karyopherin alpha, Cell, issue.2, pp.94193-204, 1998. ,
Nuclear export complexes in the frame, Current Opinion in Structural Biology, vol.20, issue.2, pp.247-252, 2010. ,
DOI : 10.1016/j.sbi.2010.01.012
The NTF2 Gene Encodes an Essential, Highly Conserved Protein That Functions in Nuclear Transport in Vivo, Journal of Biological Chemistry, vol.271, issue.31, pp.27118477-18484, 1996. ,
DOI : 10.1074/jbc.271.31.18477
Editing CCR5: A Novel Approach to HIV Gene Therapy, Advances in Experimental Medicine and Biology, vol.848, pp.117-130, 2015. ,
DOI : 10.1007/978-1-4939-2432-5_6
HIV Controllers With Different Viral Load Cutoff Levels Have Distinct Virologic and Immunologic Profiles, JAIDS Journal of Acquired Immune Deficiency Syndromes, vol.68, issue.4, pp.377-385, 1999. ,
DOI : 10.1097/QAI.0000000000000500
Overcoming pharmacologic sanctuaries, Current Opinion in HIV and AIDS, vol.8, issue.3, pp.190-195, 2013. ,
DOI : 10.1097/COH.0b013e32835fc68a
URL : http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3677586
Understanding HIV Latency: The Road to an HIV Cure, Annual Review of Medicine, vol.66, issue.1, pp.407-421, 2015. ,
DOI : 10.1146/annurev-med-092112-152941
URL : http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4381961
The protein as a variable in protein crystallization, Journal of Structural Biology, vol.142, issue.1, pp.88-97, 2003. ,
DOI : 10.1016/S1047-8477(03)00041-8
Structure, dynamics and function of nuclear pore complexes, Trends in Cell Biology, vol.18, issue.10, pp.456-466, 2008. ,
HIV Rev response element (RRE) directs assembly of the Rev homooligomer into discrete asymmetric complexes, Proceedings of the National Academy of Sciences of the United States of America, pp.12481-12486, 2010. ,
DOI : 10.1038/nprot.2008.156
Structural basis for cooperative RNA binding and export complex assembly by HIV Rev, Nature Structural & Molecular Biology, vol.276, issue.11, pp.171337-1342, 2010. ,
DOI : 10.1107/S0907444994003112
URL : http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2988976
Understanding HIV infection for the design of a therapeutic vaccine. Part I: Epidemiology and pathogenesis of HIV infection, Annales Pharmaceutiques Fran??aises, vol.73, issue.2, pp.87-99, 2015. ,
DOI : 10.1016/j.pharma.2014.11.002
The HADDOCK web server for data-driven biomolecular docking, Nature Protocols, vol.3, issue.5, pp.883-897, 2010. ,
DOI : 10.1038/nprot.2010.32
Genetic restriction of HIV-1 infection and progression to AIDS by a deletion allele of the CKR5 structural gene. Hemophilia Growth and Development Study, Multicenter AIDS Cohort Study Science, issue.5283, pp.2731856-1862, 1996. ,
Disorder in the nuclear pore complex: The FG repeat regions of nucleoporins are natively unfolded, Proceedings of the National Academy of Sciences of the United States of America, pp.2450-2455, 2003. ,
DOI : 10.1091/mbc.6.11.1591
Rational Protein Crystallization by Mutational Surface Engineering, Structure, vol.12, issue.4, pp.529-535, 1993. ,
DOI : 10.1016/j.str.2004.03.008
URL : http://doi.org/10.1016/j.str.2004.03.008
It's all in the crystals???, Acta Crystallographica Section D Biological Crystallography, vol.15, issue.4, pp.243-248, 2011. ,
DOI : 10.1107/S0907444911007797
Components of Coated Vesicles and Nuclear Pore Complexes Share a Common Molecular Architecture, PLoS Biology, vol.11, issue.12, p.380, 2004. ,
DOI : 10.1371/journal.pbio.0020380.st007
Structure of a Truncation Mutant of the Nuclear Export Factor CRM1 Provides Insights into the Auto-Inhibitory Role of Its C-Terminal Helix, Structure, vol.21, issue.8, pp.211338-1349, 1993. ,
DOI : 10.1016/j.str.2013.06.003
URL : https://hal.archives-ouvertes.fr/hal-01322366
Implications of the HIV-1 Rev dimer structure at 3.2 A resolution for multimeric binding to the Rev response element, Proceedings of the National Academy of Sciences of the United States of America, pp.5810-5814, 2010. ,
DOI : 10.1002/jcc.20084
The nucleoplasmin nuclear location sequence is larger and more complex than that of SV-40 large T antigen, The Journal of Cell Biology, vol.107, issue.3, pp.841-849, 1988. ,
DOI : 10.1083/jcb.107.3.841
Ciliary entry of the kinesin-2 motor KIF17 is regulated by importin-??2 and RanGTP, Nature Cell Biology, vol.147, issue.7, pp.703-710, 2010. ,
DOI : 10.1038/ncb2073
Long-acting HIV drugs advanced to overcome adherence challenge, Nature Medicine, vol.343, issue.4, pp.323-324, 2014. ,
DOI : 10.1038/nm0414-323
HADDOCK:?? A Protein???Protein Docking Approach Based on Biochemical or Biophysical Information, Journal of the American Chemical Society, vol.125, issue.7, pp.1251731-1737, 2003. ,
DOI : 10.1021/ja026939x
Strategies to guide the antibody affinity maturation process, Current Opinion in Virology, vol.11, pp.137-147, 2015. ,
DOI : 10.1016/j.coviro.2015.04.002
URL : http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4456294
Application of gene-editing technologies to HIV-1, Current Opinion in HIV and AIDS, vol.10, issue.2, pp.123-127, 2015. ,
DOI : 10.1097/COH.0000000000000139
Live imaging of single nuclear pores reveals unique assembly kinetics and mechanism in interphase, The Journal of Cell Biology, vol.113, issue.1, pp.15-22, 2010. ,
DOI : 10.1083/jcb.200605053
Thermofluor-based high-throughput stability optimization of proteins for structural studies, Analytical Biochemistry, vol.357, issue.2, pp.289-298, 2006. ,
DOI : 10.1016/j.ab.2006.07.027
Timing of Intermittent Seminal HIV-1 RNA Shedding in Patients with Undetectable Plasma Viral Load under Combination Antiretroviral Therapy, PLoS ONE, vol.25, issue.16, p.88922, 2014. ,
DOI : 10.1371/journal.pone.0088922.s001
Structural basis of recognition of monopartite and bipartite nuclear localization sequences by mammalian importin-??11Edited by K. Nagai, Journal of Molecular Biology, vol.297, issue.5, pp.1183-1194, 2000. ,
DOI : 10.1006/jmbi.2000.3642
Structural basis for the specificity of bipartite nuclear localization sequence binding by importin-alpha, The Journal of Biological Chemistry, issue.30, pp.27827981-27987, 2003. ,
Nuclear import pathway of the telomere elongation suppressor TRF1 : inhibition by importin alpha, Biochemistry, issue.30, pp.419333-9340, 2002. ,
Nuclear import of Creb and AP-1 transcription factors requires importin-beta 1 and Ran but is independent of importin-alpha, Biochemistry, issue.17, pp.405208-5217, 2001. ,
Quantitative Structural Analysis of Importin-?? Flexibility: Paradigm for Solenoid Protein Structures, Structure, vol.18, issue.9, pp.181171-1183, 1993. ,
DOI : 10.1016/j.str.2010.06.015
Kap95p Binding Induces the Switch Loops of RanGDP to Adopt the GTP-Bound Conformation: Implications for Nuclear Import Complex Assembly Dynamics, Journal of Molecular Biology, vol.383, issue.4, pp.772-782, 2008. ,
DOI : 10.1016/j.jmb.2008.07.090
Investigating Dengue Virus Nonstructural Protein 5 (NS5) Nuclear Import, Methods in Molecular Biology, vol.1138, pp.301-328, 2014. ,
DOI : 10.1007/978-1-4939-0348-1_19
Conformational Variability of Nucleo-cytoplasmic Transport Factors, Journal of Biological Chemistry, vol.279, issue.3, pp.2176-2181, 2004. ,
DOI : 10.1074/jbc.M309112200
Regulation of nucleocytoplasmic trafficking of viral proteins: An integral role in pathogenesis?, Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, vol.1813, issue.12, pp.18132176-2190, 2011. ,
DOI : 10.1016/j.bbamcr.2011.03.019
Concentrations of TFV-DP/FTC-TP in dried blood spots and TFV/FTC in hair are strongly correlated in iPrEx OLE : Implications for PrEP adherence monitoring, M., and iPrEX study team, 2015. ,
A Versatile and Efficient High-Throughput Cloning Tool for Structural Biology, Biochemistry, vol.50, issue.15, pp.503272-3278, 2011. ,
DOI : 10.1021/bi200178z
Toward rational protein crystallization: A Web server for the design of crystallizable protein variants, Protein Science, vol.103, issue.2, pp.161569-1576, 2007. ,
DOI : 10.1110/ps.072914007
Exportin 4 mediates a novel nuclear import pathway for Sox family transcription factors, The Journal of Cell Biology, vol.19, issue.1, pp.27-34, 2009. ,
DOI : 10.1083/jcb.152.4.729
URL : http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2700522
A Novel Class of RanGTP Binding Proteins, The Journal of Cell Biology, vol.13, issue.1, pp.65-80, 1997. ,
DOI : 10.1038/376184a0
Identification of different roles for RanGDP and RanGTP in nuclear protein import, The EMBO Journal, issue.20, pp.155584-5594, 1996. ,
Uptake of pre-exposure prophylaxis, sexual practices, and HIV incidence in men and transgender women who have sex with men: a cohort study, and iPrEx study team, pp.820-829, 2014. ,
DOI : 10.1016/S1473-3099(14)70847-3
Functional Architecture of the Nuclear Pore Complex, Annual Review of Biophysics, vol.41, issue.1, pp.557-584, 2012. ,
DOI : 10.1146/annurev-biophys-050511-102328
Intermolecular masking of the HIV-1 Rev NLS by the cellular protein HIC: Novel insights into the regulation of Rev nuclear import, Retrovirology, vol.8, issue.1, p.17, 2011. ,
DOI : 10.1083/jcb.111.3.807
Genome editing-based HIV therapies, Trends in Biotechnology, vol.33, issue.3, pp.172-179, 2015. ,
DOI : 10.1016/j.tibtech.2014.12.006
NES consensus redefined by structures of PKI-type and Rev-type nuclear export signals bound to CRM1, Nature Structural & Molecular Biology, vol.36, issue.11, pp.171367-1376, 2010. ,
DOI : 10.1093/emboj/cdg565
MD Simulations and FRET Reveal an Environment-Sensitive Conformational Plasticity of Importin-??, Biophysical Journal, vol.109, issue.2, pp.277-286, 2015. ,
DOI : 10.1016/j.bpj.2015.06.014
A controlled trial of two nucleoside analogues plus indinavir in persons with human immunodeficiency virus infection and CD4 cell counts of 200 per cubic millimeter or less. AIDS Clinical Trials Group 320 Study Team, pp.337725-733, 1997. ,
HIV-1 RNAs, Current Opinion in HIV and AIDS, vol.10, issue.2, pp.103-109, 2015. ,
DOI : 10.1097/COH.0000000000000135
Interactions between HIV rev and nuclear import and export factors: the rev nuclear localisation signal mediates specific binding to human importin-??, Journal of Molecular Biology, vol.274, issue.5, pp.274693-707, 1997. ,
DOI : 10.1006/jmbi.1997.1420
GTP Hydrolysis by Ran Is Required for Nuclear Envelope Assembly, Molecular Cell, vol.5, issue.6, pp.1013-1024, 2000. ,
DOI : 10.1016/S1097-2765(00)80266-X
URL : http://doi.org/10.1016/s1097-2765(00)80266-x
The Structure of the Nuclear Pore Complex, Annual Review of Biochemistry, vol.80, issue.1, pp.613-643, 2011. ,
DOI : 10.1146/annurev-biochem-060109-151030
The importin-?? binding domain of snurportin1 is responsible for the Ran- and energy-independent nuclear import of spliceosomal U snRNPs in vitro, The Journal of Cell Biology, vol.15, issue.3, pp.467-479, 2002. ,
DOI : 10.1126/science.7754385
Long-term control of HIV by CCR5 Delta32/Delta32 stem-cell transplantation, pp.692-698, 2009. ,
Nuclear pore proteins and the control of genome functions, Genes & Development, vol.29, issue.4, pp.337-349, 2015. ,
DOI : 10.1101/gad.256495.114
Recognition of the high affinity binding site in rev-response element RNA by the Human Immunodeficiency Virus type-1 rev protein, Nucleic Acids Research, vol.20, issue.24, pp.6465-6472, 1992. ,
DOI : 10.1093/nar/20.24.6465
Structural Model for the Cooperative Assembly of HIV-1 Rev Multimers on the RRE as Deduced from Analysis of Assembly-Defective Mutants, Molecular Cell, vol.7, issue.3, pp.603-614, 2001. ,
DOI : 10.1016/S1097-2765(01)00207-6
The importin ??/importin 7 heterodimer is a functional nuclear import receptor for histone H1, The EMBO Journal, vol.18, issue.9, pp.2411-2423, 1999. ,
DOI : 10.1093/emboj/18.9.2411
Nuclear Pore Complex, International Review of Cell and Molecular Biology, vol.287, pp.233-286, 2011. ,
DOI : 10.1016/B978-0-12-386043-9.00006-2
RNAdirected remodeling of the HIV-1 protein Rev orchestrates assembly of the Rev-Rev response element complex, eLife, vol.3, p.4120, 2014. ,
Multi-Faceted Post-Transcriptional Functions of HIV-1 Rev, Biology, vol.12, issue.3, pp.165-174, 2012. ,
DOI : 10.1016/S1054-3589(07)55001-5
The nuclear pore complex - structure and function at a glance, Journal of Cell Science, vol.128, issue.3, pp.423-429, 2015. ,
DOI : 10.1242/jcs.083246
Subunits of the heterotrimeric transcription factor NF-Y are imported into the nucleus by distinct pathways involving importin beta and importin 13, Molecular and Cellular Biology, issue.13, pp.255339-5354, 2005. ,
Sequence requirements for nuclear location of simian virus 40 large-T antigen, Nature, vol.292, issue.5981, pp.31133-31171, 1984. ,
DOI : 10.1038/311033a0
An Unusual Hydrophobic Core Confers Extreme Flexibility to HEAT Repeat Proteins, Biophysical Journal, vol.99, issue.5, pp.991596-1603, 2010. ,
DOI : 10.1016/j.bpj.2010.06.032
URL : http://doi.org/10.1016/j.bpj.2010.06.032
Antiretroviral Therapy in the Elite Controller : Justified or Premature ? The Journal of Infectious Diseases, 2014. ,
Biological Significance of the Importin-?? Family-Dependent Nucleocytoplasmic Transport Pathways, Traffic, vol.288, issue.Suppl. 1, pp.15727-748, 2014. ,
DOI : 10.1111/tra.12174
Autoinhibition by an internal nuclear localization signal revealed by the crystal structure of mammalian importin alpha, Nature Structural Biology, vol.6, issue.4, pp.388-397, 1999. ,
DOI : 10.1038/7625
When protein folding is simplified to protein coiling: the continuum of solenoid protein structures, Trends in Biochemical Sciences, vol.25, issue.10, pp.25509-515, 2000. ,
DOI : 10.1016/S0968-0004(00)01667-4
Virus strategies for passing the nuclear envelope barrier, Nucleus, vol.70, issue.6, pp.3526-539, 2012. ,
DOI : 10.1128/mBio.00278-11
Six Classes of Nuclear Localization Signals Specific to Different Binding Grooves of Importin ??, Journal of Biological Chemistry, vol.284, issue.1, pp.478-485, 2009. ,
DOI : 10.1074/jbc.M807017200
Conformational Propensities of Intrinsically Disordered Proteins from NMR Chemical Shifts, ChemPhysChem, vol.52, issue.13, pp.143034-3045, 2013. ,
DOI : 10.1002/cphc.201300387
URL : https://hal.archives-ouvertes.fr/hal-01321617
Molecular Dissection of the Importin ??1-Recognized Nuclear Targeting Signal of Parathyroid Hormone-Related Protein, Biochemical and Biophysical Research Communications, vol.282, issue.2, pp.629-634, 2001. ,
DOI : 10.1006/bbrc.2001.4607
HIV Controllers: A Homogeneous Group of HIV-1--Infected Patients with Spontaneous Control of Viral Replication, Clinical Infectious Diseases, vol.41, issue.7, pp.411053-1056, 2005. ,
DOI : 10.1086/433188
HIV Controllers: A Homogeneous Group of HIV-1--Infected Patients with Spontaneous Control of Viral Replication, Clinical Infectious Diseases, vol.41, issue.7, pp.54566-571, 2006. ,
DOI : 10.1086/433188
Nuclear Entry of High-Risk Human Papillomavirus Type 16 E6 Oncoprotein Occurs via Several Pathways, Journal of Virology, vol.77, issue.4, pp.2330-2337, 2003. ,
DOI : 10.1128/JVI.77.4.2330-2337.2003
The adoption of a twisted structure of importin-?? is essential for the protein-protein interaction required for nuclear transport, Journal of Molecular Biology, vol.302, issue.1, pp.251-264, 2000. ,
DOI : 10.1006/jmbi.2000.4055
Structural basis for nuclear import complex dissociation by RanGTP, Nature, vol.22, issue.7042, pp.435693-696, 2005. ,
DOI : 10.1006/jmbi.1998.2204
The structure of importinbeta bound to SREBP-2 : nuclear import of a transcription factor, Science, issue.5650, pp.3021571-1575, 2003. ,
A set of BEST triple-resonance experiments for time-optimized protein resonance assignment, Journal of Magnetic Resonance, vol.187, issue.1, pp.163-169, 1997. ,
DOI : 10.1016/j.jmr.2007.04.002
Nucleocytoplasmic shuttling of HIV-1 integrase is controlled by the viral Rev protein, Nucleus, vol.66, issue.2, pp.190-201, 2010. ,
DOI : 10.1016/j.virusres.2006.02.009
Transportin 3 and importin ?? are required for effective nuclear import of HIV-1 integrase in virus-infected cells, Nucleus, vol.66, issue.5, pp.422-431, 2010. ,
DOI : 10.1038/87979
Strategies to inhibit viral protein nuclear import: HIV-1 as a target, Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, vol.1813, issue.9, pp.18131646-1653, 2011. ,
DOI : 10.1016/j.bbamcr.2010.07.010
URL : http://doi.org/10.1016/j.bbamcr.2010.07.010
Inhibition of HIV-1 infection of primary CD4+ T-cells by gene editing of CCR5 using adenovirus-delivered CRISPR/Cas9, Journal of General Virology, vol.96, issue.8, 2015. ,
DOI : 10.1099/vir.0.000139
Homozygous Defect in HIV-1 Coreceptor Accounts for Resistance of Some Multiply-Exposed Individuals to HIV-1 Infection, Cell, vol.86, issue.3, pp.367-377, 1996. ,
DOI : 10.1016/S0092-8674(00)80110-5
A Molecular Rheostat, Journal of Molecular Biology, vol.241, issue.2, pp.193-207, 1994. ,
DOI : 10.1006/jmbi.1994.1488
Rapid and efficient purification of RNA-binding proteins: Application to HIV-1 Rev, Protein Expression and Purification, vol.63, issue.2, pp.112-119, 2009. ,
DOI : 10.1016/j.pep.2008.09.010
Molecular basis for specificity of nuclear import and prediction of nuclear localization, Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, vol.1813, issue.9, pp.18131562-1577, 2011. ,
DOI : 10.1016/j.bbamcr.2010.10.013
Combining methods for speeding up multi-dimensional acquisition. Sparse sampling and fast pulsing methods for unfolded proteins, Journal of Magnetic Resonance, vol.206, issue.1, pp.81-87, 1997. ,
DOI : 10.1016/j.jmr.2010.06.007
TIME SEQUENCE OF NUCLEAR PORE FORMATION IN PHYTOHEMAGGLUTININ-STIMULATED LYMPHOCYTES AND IN HELA CELLS DURING THE CELL CYCLE, The Journal of Cell Biology, vol.55, issue.2, pp.433-447, 1972. ,
DOI : 10.1083/jcb.55.2.433
Structural characterization of proteins and complexes using small-angle X-ray solution scattering, Journal of Structural Biology, vol.172, issue.1, pp.128-141, 2010. ,
DOI : 10.1016/j.jsb.2010.06.012
Molecular basis for the recognition of snurportin 1 by importin beta, The Journal of Biological Chemistry, issue.12, pp.2837877-7884, 2008. ,
HIV-1 remodels the nuclear pore complex, The Journal of Cell Biology, vol.73, issue.4, pp.619-631, 2011. ,
DOI : 10.1083/jcb.201008064.dv
Nuclear Import of Cdk/Cyclin Complexes: Identification of Distinct Mechanisms for Import of Cdk2/Cyclin E and Cdc2/Cyclin B1, The Journal of Cell Biology, vol.13, issue.2, pp.213-224, 1999. ,
DOI : 10.1101/gad.12.14.2131
The GTP-binding protein Ran/TC4 is required for protein import into the
nucleus, Nature, vol.365, issue.6447, pp.365661-663, 1993. ,
DOI : 10.1038/365661a0
Nuclear Trafficking in Health and Disease, Current Opinion in Cell Biology, vol.28, pp.28-35, 2014. ,
DOI : 10.1016/j.ceb.2014.01.007
URL : http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4061247
ign: An Assignment Strategy for Maximizing Information from the Mass Spectra of Heterogeneous Protein Assemblies, Analytical Chemistry, vol.84, issue.6, pp.2939-2948, 2012. ,
DOI : 10.1021/ac300056a
Previously identified protein of uncertain function is karyopherin alpha and together with karyopherin beta docks import substrate at nuclear pore complexes., Proceedings of the National Academy of Sciences, vol.92, issue.6, pp.922008-2011, 1995. ,
DOI : 10.1073/pnas.92.6.2008
Karyopherins: from nuclear-transport mediators to nuclear-function regulators, Trends in Cell Biology, vol.14, issue.10, pp.547-556, 2004. ,
DOI : 10.1016/j.tcb.2004.09.004
Multiple pathways contribute to nuclear import of core histones, EMBO reports, vol.14, issue.8, pp.690-696, 2001. ,
DOI : 10.1093/embo-reports/kve168
Molecular strategies to inhibit HIV-1 replication, Retrovirology, vol.2, issue.1, p.10, 2005. ,
DOI : 10.1186/1742-4690-2-10
The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability, Nature Protocols, vol.4, issue.9, pp.2212-2221, 2007. ,
DOI : 10.1038/nprot.2006.202
Viral infection: CRISPR???Cas9 defence against HIV-1, Nature Reviews Microbiology, vol.13, issue.5, p.250, 2015. ,
DOI : 10.1038/nrmicro3474
Comparing HIV-1 and HIV-2 infection: Lessons for viral immunopathogenesis, Reviews in Medical Virology, vol.18, issue.Suppl 2, pp.221-240, 2013. ,
DOI : 10.1002/rmv.1739
Self-Organization of Microtubule Asters Induced in Xenopus Egg Extracts by GTP-Bound Ran, Science, vol.284, issue.5418, pp.2841356-1358, 1999. ,
DOI : 10.1126/science.284.5418.1356
Comparative study of protein molecular weights by size-exclusion chromatography and laser-light scattering, Journal of Pharmaceutical and Biomedical Analysis, vol.25, issue.5-6, pp.5-6833, 2001. ,
DOI : 10.1016/S0731-7085(01)00359-4
Strategies for Improving Crystallization Success Rates, Methods in Molecular Biology, vol.426, pp.345-362, 2008. ,
DOI : 10.1007/978-1-60327-058-8_22
Importin ?? Can Mediate the Nuclear Import of an Arginine-Rich Nuclear Localization Signal in the Absence of Importin ??, Molecular and Cellular Biology, vol.19, issue.2, pp.1218-1225, 1999. ,
DOI : 10.1128/MCB.19.2.1218
Antiretroviral Therapy, Infectious Disease Clinics of North America, vol.28, issue.3, pp.371-402, 2014. ,
DOI : 10.1016/j.idc.2014.06.001
Lower self-reported Quality of Life in HIV-infected patients on cART and with low co-morbidity compared to healthy controls, Journal of Acquired Immune Deficiency Syndromes, 1999. ,
New developments in the ATSAS program package for small-angle scattering data analysis, Journal of Applied Crystallography, pp.45342-350, 2012. ,
Epstein-Barr Virus??? and Kaposi Sarcoma-Associated Herpesvirus???Related Malignancies in the Setting of Human Immunodeficiency Virus Infection, Seminars in Oncology, vol.42, issue.2, pp.258-271, 2015. ,
DOI : 10.1053/j.seminoncol.2014.12.026
Health-related quality of life in French people living with HIV in 2003: results from the national ANRS-EN12-VESPA Study, AIDS, vol.21, issue.Suppl 1, pp.19-27, 2007. ,
DOI : 10.1097/01.aids.0000255081.24105.d7
Diversification of importin-?? isoforms in cellular trafficking and disease states, Biochemical Journal, vol.103, issue.1, pp.13-28, 2015. ,
DOI : 10.1128/JVI.00371-07
Viral load and heterosexual transmission of human immunodeficiency virus type 1. Rakai Project Study Group, pp.342921-929, 2000. ,
DOI : 10.1056/nejm200003303421303
The long-acting integrase inhibitor GSK744 protects macaques from repeated intravaginal SHIV challenge, Science Translational Medicine, vol.2, issue.14, pp.270-275, 2015. ,
DOI : 10.1126/scitranslmed.3000391
Mass spectrometric methods to analyze the structural organization of macromolecular complexes, Methods, vol.89, 2015. ,
DOI : 10.1016/j.ymeth.2015.03.004
The ethics of talking about 'HIV cure'. BMC medical ethics, p.18, 2015. ,
Kinetic analysis of translocation through nuclear pore complexes, The EMBO Journal, vol.20, issue.6, pp.1320-1330, 2001. ,
DOI : 10.1093/emboj/20.6.1320
Partners of people on ART - a New Evaluation of the Risks (The PARTNER study): design and methods, and PARTNER study group, p.296, 2012. ,
DOI : 10.1097/01.qai.0000157389.78374.45
Label-free characterization of carbonic anhydrase-novel inhibitor interactions using surface plasmon resonance, isothermal titration calorimetry and fluorescence-based thermal shift assays, Journal of Molecular Recognition, vol.10, issue.1, pp.46-56, 2014. ,
DOI : 10.1002/jmr.2330
Resistance to HIV-1 infection in Caucasian individuals bearing mutant alleles of the CCR-5 chemokine receptor gene, Nature, vol.382, issue.6593, pp.382722-725, 1996. ,
DOI : 10.1038/382722a0
NMR solution structure of the RNA-binding peptide from human immunodeficiency virus (type 1) Rev, Biochemistry, issue.26, pp.348242-8249, 1995. ,
The Crystal Structure of the Ran-Nup153ZnF2 Complex: a General Ran Docking Site at the Nuclear Pore Complex, Structure, vol.16, issue.7, pp.161116-1125, 1993. ,
DOI : 10.1016/j.str.2008.03.014
Probing allosteric mechanisms using native mass spectrometry, Current Opinion in Structural Biology, vol.34, pp.7-16, 2015. ,
DOI : 10.1016/j.sbi.2015.05.002
Origins of HIV and the AIDS Pandemic, Cold Spring Harbor Perspectives in Medicine, vol.1, issue.1, p.6841, 2011. ,
DOI : 10.1101/cshperspect.a006841
Chemical cross-linking and native mass spectrometry: A fruitful combination for structural biology, Protein Science, vol.11, issue.8, 2015. ,
DOI : 10.1002/pro.2696
A Tandem Mass Spectrometer for Improved Transmission and Analysis of Large Macromolecular Assemblies, Analytical Chemistry, vol.74, issue.6, pp.741402-1407, 2002. ,
DOI : 10.1021/ac0110552
BEST-TROSY experiments for time-efficient sequential resonance assignment of large disordered proteins, Journal of Biomolecular NMR, vol.17, issue.4, pp.311-321, 2013. ,
DOI : 10.1007/s10858-013-9715-0
URL : https://hal.archives-ouvertes.fr/hal-01321583
Potent knock down of HIV-1 replication by targeting HIV-1 Tat/Rev RNA sequences synergistically with catalytic RNA and DNA, AIDS, vol.21, issue.1, pp.31-40, 2007. ,
DOI : 10.1097/QAD.0b013e3280118fb6
GSK1265744 Pharmacokinetics in Plasma and Tissue After Single-Dose Long-Acting Injectable Administration in Healthy Subjects, JAIDS Journal of Acquired Immune Deficiency Syndromes, vol.67, issue.5, pp.67481-486, 1999. ,
DOI : 10.1097/QAI.0000000000000301
High-throughput protein crystallization, Current Opinion in Structural Biology, vol.10, issue.5, pp.558-563, 2000. ,
DOI : 10.1016/S0959-440X(00)00131-7
HIV-1 pathogenesis, Nature Medicine, vol.9, issue.7, pp.853-860, 2003. ,
DOI : 10.1038/nm0703-853
Molecular mechanism of the nuclear protein import cycle, Nature Reviews Molecular Cell Biology, vol.415, issue.3, pp.195-208, 2007. ,
DOI : 10.1038/nrm2114
The structure of the Q69L mutant of GDP-ran shows a major conformational change in the switch II loop that accounts for its failure to bind nuclear transport factor 2 (NTF2), Journal of Molecular Biology, vol.284, issue.5, pp.1517-1527, 1998. ,
DOI : 10.1006/jmbi.1998.2204
Minimal nuclear pore complexes define FG repeat domains essential for transport, Nature Cell Biology, vol.6, issue.3, pp.197-206, 2004. ,
DOI : 10.1038/ncb1097
Ran-independent nuclear import of cyclin B1-Cdc2 by importin beta, Proceedings of the National Academy of Sciences of the United States of America, issue.14, pp.967938-7943, 1999. ,
Flexible Gates: Dynamic Topologies and Functions for FG Nucleoporins in Nucleocytoplasmic Transport, Eukaryotic Cell, vol.8, issue.12, pp.1814-1827, 2009. ,
DOI : 10.1128/EC.00225-09
URL : http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2794212
Aging and HIV/AIDS: pathogenetic role of therapeutic side effects, Laboratory Investigation, vol.13, issue.2, pp.120-128, 2014. ,
DOI : 10.1097/COH.0b013e3283562a27
The Arginine-Rich Domains Present in Human Immunodeficiency Virus Type 1 Tat and Rev Function as Direct Importin ??-Dependent Nuclear Localization Signals, Molecular and Cellular Biology, vol.19, issue.2, pp.1210-1217, 1999. ,
DOI : 10.1128/MCB.19.2.1210
Structural view of the Ran-Importin beta interaction at 2.3 A resolution, Cell, issue.5, pp.97635-646, 1999. ,
Structure of a Ran-binding domain complexed with Ran bound to a GTP analogue : implications for nuclear transport, Nature, issue.6722, pp.39839-39885, 1999. ,
HIV and the Gut Microbiota, Partners in Crime: Breaking the Vicious Cycle to Unearth New Therapeutic Targets, Journal of Immunology Research, vol.124, issue.6, p.614127, 2015. ,
DOI : 10.1371/journal.ppat.1003890
Nuclear Translocation and DNA Recognition Signals Colocalized within the bZIP Domain of Cyclic Adenosine 3???,5???-Monophosphate Response Element-Binding Protein CREB, Molecular Endocrinology, vol.5, issue.10, pp.51431-1438, 1991. ,
DOI : 10.1210/mend-5-10-1431
Ivermectin is a specific inhibitor of importin ??/??-mediated nuclear import able to inhibit replication of HIV-1 and dengue virus, Biochemical Journal, vol.44, issue.3, pp.443851-856, 2012. ,
DOI : 10.1039/c0cs00092b
Nuclear Import of c-Jun Is Mediated by Multiple Transport Receptors, Journal of Biological Chemistry, vol.282, issue.38, pp.28227685-27692, 2007. ,
DOI : 10.1074/jbc.M703301200
RanGTP mediates nuclear pore complex assembly, Nature, vol.424, issue.6949, pp.424689-694, 2003. ,
DOI : 10.1038/nature01898
An Efficient and Highly Diastereoselective Synthesis of GSK1265744, a Potent HIV Integrase Inhibitor, Organic Letters, vol.17, issue.3, pp.564-567, 2015. ,
DOI : 10.1021/ol503580t
Probability-based protein secondary structure identification using combined NMR chemical-shift data, Protein Science, vol.11, issue.4, pp.852-861, 2002. ,
DOI : 10.1110/ps.3180102
URL : http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2373532
The Nuclear Pore Complex and Nuclear Transport, Cold Spring Harbor Perspectives in Biology, vol.2, issue.10, p.562, 2010. ,
DOI : 10.1101/cshperspect.a000562
Structure-Function Relationships of the G Domain, a Canonical Switch Motif, Annual Review of Biochemistry, vol.80, issue.1, pp.943-971, 2011. ,
DOI : 10.1146/annurev-biochem-062708-134043
Adenovirus Core Protein pVII Is Translocated into the Nucleus by Multiple Import Receptor Pathways, Journal of Virology, vol.80, issue.19, pp.809608-9618, 2006. ,
DOI : 10.1128/JVI.00850-06
URL : http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1617226
Structural Basis for RanGTP Independent Entry of Spliceosomal U snRNPs into the Nucleus, Journal of Molecular Biology, vol.374, issue.4, pp.1129-1138, 2007. ,
DOI : 10.1016/j.jmb.2007.09.065
AIDS therapy. First tentative signs of therapeutic promise, Nature, vol.323, issue.6086, p.283, 1986. ,
Nup358, a Cytoplasmically Exposed Nucleoporin with Peptide Repeats, Ran-GTP Binding Sites, Zinc Fingers, a Cyclophilin A Homologous Domain, and a Leucine-rich Region, Journal of Biological Chemistry, vol.270, issue.23, pp.27014209-14213, 1995. ,
DOI : 10.1074/jbc.270.23.14209
A distinct nuclear localization signal in the N terminus of Smad 3 determines its ligand-induced nuclear translocation, Proceedings of the National Academy of Sciences, vol.274, issue.47, pp.977853-7858, 2000. ,
DOI : 10.1074/jbc.274.47.33531
Recognition of nuclear targeting signals by Karyopherin-?? proteins, Current Opinion in Structural Biology, vol.20, issue.6, pp.782-790, 2010. ,
DOI : 10.1016/j.sbi.2010.09.008
How Well Can We Understand Large-Scale Protein Motions Using Normal Modes of Elastic Network Models?, Biophysical Journal, vol.93, issue.3, pp.920-929, 2007. ,
DOI : 10.1529/biophysj.106.095927
Probing the specificity of binding to the major nuclear localization sequence-binding site of importin-alpha using oriented peptide library screening, The Journal of Biological Chemistry, issue.26, pp.28519935-19946, 2010. ,
Importin-??: Structural and Dynamic Determinants of a Molecular Spring, Structure, vol.16, issue.6, pp.906-915, 1993. ,
DOI : 10.1016/j.str.2008.03.007
Chromatin-Independent Nuclear Envelope Assembly Induced by Ran GTPase in Xenopus Egg Extracts, Science, vol.288, issue.5470, pp.2881429-1432, 2000. ,
DOI : 10.1126/science.288.5470.1429
Fluorescence-based thermal shift assays, Current Opinion in Drug Discovery & Development, vol.13, issue.4, pp.389-402, 2010. ,
Overcoming the solubility limit with solubility-enhancement tags: successful applications in biomolecular NMR studies, Journal of Biomolecular NMR, vol.42, issue.1, pp.23-31, 2010. ,
DOI : 10.1007/s10858-009-9371-6
The CRISPR/Cas9 system inactivates latent HIV-1 proviral DNA, Retrovirology, vol.12, issue.1, p.22, 2015. ,
DOI : 10.1073/pnas.1405186111
URL : http://doi.org/10.1186/s12977-015-0150-z
SIDA et des hépatites virales : AIDES. Comme pour les pompiers, nous utilisons le terme de "volontaire" plutôt que celui de "bénévole". Cette association, créée par Daniel Defert en 1984, a participé entre autre, à la création des lignes d'écoute téléphonique pour soutenir les personnes séro-concernées (déléguées désormais principalement à Sida Info Service (SIS)) ainsi que la création de l'université des jeunes chercheurs (UJC, désormais maintenue difficilement par le Sidaction) Cette université est une formation intensive et enrichissante d'excellence qui a lieu tous les deux ans et dont les jeunes chercheurs francophones peuvent jouir et bénéficier. Il s'agit notament de cours de la part des ténors francophones de la recherche sur le VIH/SIDA. Via AIDES, j'ai eu une faible activité au sein du COREVIH (un comité régional de lutte contre le VIH/SIDA et d'autres agents infectieux) J'ai par exemple participé à l'établissement de tests diagnostiques d'infections sexuellement transmissibles ,
J'ai pu également collaborer sous la supervision deAIDES), puis Daniela Rojas Castro (AIDES) et Marie Préau (GRePS) au projet de recherche en sciences sociales QualiPEP, qui grâce à des 1. ? : inconnu-e 2. -TEV-: Site de cleavage à la protéase ,
Ampicilline 7 Kana : Kanamycine 8. FX cloning fait référence à la stratégie de clonage (Geertsma and Dutzler, 2011. ,