Etude de l'interaction entre la protéine humaine Importin beta et la protéine du VIH-1 Rev

Abstract : HIV-1 Rev is a small protein which mediates the nuclear export of unspliced and partially spliced viral transcripts. The nuclear import of Rev is mediated by the host cell protein Importin β (Impβ), a transport factor that recognizes the arginine- rich nuclear localization signal (NLS) present on Rev. The goal of this project is to determine the three-dimensional structure of the complex formed by Impβ and Rev. Because attempts to crystallize this complex were unsuccessful, we sought structural information using a range of biochemical, biophysical and computational approaches. The results suggest a limited number of hypothetical configurations for the Impβ/Rev complex, which can be specifically verified with additional experiments. The work described in this thesis increases our understanding of how Impβ recognizes Rev and should facilitate future efforts at determining the high-resolution structure of this complex.
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Didier Spittler. Etude de l'interaction entre la protéine humaine Importin beta et la protéine du VIH-1 Rev. Biochimie, Biologie Moléculaire. Université Grenoble Alpes, 2015. Français. ⟨NNT : 2015GREAV034⟩. ⟨tel-01498398⟩

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