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Etude de la formation du duramen chez le douglas : approches biochimique et transcriptomique

Abstract : The heartwood formation is a key physiological process involved in wood quality because it contributes to its natural durability. The goal of this thesis is to understand mechanisms involved in the heartwood formation in douglas fir. This study has been carried out at phenotypic, biochemical and molecular levels from several douglas-fir genotypes. Thanks to phenotypic analysis, we showed that heartwood proportion is probably under genetic control, and little influenced by the environment. In douglas fir, heartwood expansion mainly occurs during autumn and winter. To characterize the biochemical composition of wood, a new method has been developed. The method implies the wood dissolution in ionic liquid, the solution obtained are then analyzed by immunodetection with monoclonal antibodies against plant cell wall glycan epitopes. Thanks to this method, the wood cell wall composition has been studied, ring-by-ring, from the outer sapwood to the inner heartwood. Some polymers are more abundant in the sapwood (arabinans) while others in the heartwood (pectins, xylans and galactans). Then, genes involved in the heartwood formation have been studied by RNA-Seq from trees belonging to one genotype sampled during winter. Results show that genes encoding transcription factors, defence related proteins, enzymes involved in phenylpropanoid biosynthesis and plant cell wall modification are upregulated in transition zone compared to sapwood. Hormones, ethylene and jasmonate especially, seem to play an important role during sapwood maturation.
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Submitted on : Wednesday, March 1, 2017 - 4:36:11 PM
Last modification on : Sunday, June 26, 2022 - 1:22:53 PM
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  • HAL Id : tel-01480671, version 1



Idelette Plazanet. Etude de la formation du duramen chez le douglas : approches biochimique et transcriptomique. Autre. Université de Limoges, 2016. Français. ⟨NNT : 2016LIMO0101⟩. ⟨tel-01480671⟩



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