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Rôle de la protéine chaperonne Hfq dans la réponse des ARN messagers à la régulation par les petits ARN

Benoit Guillemardet 1 
1 RGSP - Régulation génétique chez Salmonella et ses phages
DBG - Département Biologie des Génomes
Abstract : Among the nucleic regulators, small regulatory RNAs are a quick way to adapt. They are found in all areas of life. In bacteria, the action of a large number of them depends on the chaperone protein of Hfq RNA. Their mode of action consists of matching directly to the target mRNA by imperfect complementary sequence. This results in a positive or negative control, translation and/or stability of the target mRNA.During the first part of this thesis, we have tried to characterize the regulation of mRNA by small regulatory RNAs. To do this, we studied the different components of this regulation and we have shown that the binding sites on protein Hfq on RNA should be consistent for optimal regulation of mRNA by small regulatory RNAs.In the second part of the thesis, we identified a new type of mRNA regulation by small RNA regulators: transcriptional polarity. We could show that the fixing of small RNA ChiX on the 5 'UTR region of the mRNA chiPQ cause inhibition of translation of chiP but also a Rho-dependent premature termination of mRNA transcription.We subsequently investigated whether this type of regulation could be in the control of other mRNAs as dppABCDF’s operon and pgaABCD’s operon. In parallel with these studies, we have also tried characterized Rho-dependent termination between S. Typhimurium and E. coli in mRNA chiPQ.
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Submitted on : Tuesday, November 8, 2016 - 12:41:12 PM
Last modification on : Saturday, June 25, 2022 - 8:27:53 PM
Long-term archiving on: : Tuesday, March 14, 2017 - 11:07:14 PM

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  • HAL Id : tel-01393921, version 1

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Benoit Guillemardet. Rôle de la protéine chaperonne Hfq dans la réponse des ARN messagers à la régulation par les petits ARN. Biochimie, Biologie Moléculaire. Université Paris-Saclay, 2016. Français. ⟨NNT : 2016SACLS226⟩. ⟨tel-01393921⟩

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