Design and fabrication of advanced microdevices for stem cell and cancer cell studies

Abstract : This work aimed to provide new tools and methods that can be used for advanced studies of stem cells and cancer cells. We first developed a patch method for off-ground culture and differentiation of human induced pluripotent stem cells (hiPSCs). The culture patch we proposed consists of crosslinked monolayer gelatin nanofibers on a honeycomb frame to ensure minimal exogenous material contact and maximum permeability. Then, we demonstrated the formation of cardiac tissue constructs and motor neurons on the patch, started from embryoid-body like and monolayer hiPSC colonies, respectively. We also developed a microfluidic device with integrated filter for isolation of circulating tumor cells (CTCs), showing high capture performances in terms of efficiency, selectivity and cell viability. Finally, we evaluated the anti-cancer drug effect on the formation of tumor spheroids by using microfabricated agarose multi-wells. All together, we progressed in micro-engineering toward large scale applications.
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Yadong Tang. Design and fabrication of advanced microdevices for stem cell and cancer cell studies. Analytical chemistry. Université Pierre et Marie Curie - Paris VI, 2016. English. ⟨NNT : 2016PA066035⟩. ⟨tel-01358773⟩

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