Synthèse et caractérisations de nanoparticules d’or pour la vectorisation de principes actifs anticancéreux

Abstract : Today, in the anti-cancerous treatment, the increase of the cytotoxic activity of natural active compounds and the decrease of side-effects during the treatment remain one of the greatest medical problems. In this work, two natural active compounds, which have great interest in anticancer-applications, are extracted from plants. To increase their water-solubility, a new drug delivery nanovector based on gold nanoparticles has been synthesized with several steps. The nano-drug-delivery-vector, between 30 and 200 nm, can be concentrated to the tumor tissue only, by EPR effect. The nanovector is built on a core of gold nanoparticles covered by PEI, bearing anchored cyclodextrin, in which the drug is encapsulated. Accidental killing of healthy cells by the drug is avoided by this way. The system is characterized by IR, UV-Visible, GTA, TEM, DLS and zeta potential. “in vitro” tests are realized on two prostate cancer cell-lines PC-3, DU-145 and two colon cancer lines HT29, HCT116. The results show an improvement of the activity of the natural compound by using gold nanovector. The same system is used to deliver a photosensitizer. A chlorin obtained from chlorophyll a is fixed onto the surface of spherical or triangular nanoparticles. The same characterizations are used as the former delivery nanosystem. Their photocytotoxic effet is tested on HT29 cancer line. An improvement of the activity by using nanoparticles is also observed.
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Shihong Qiu. Synthèse et caractérisations de nanoparticules d’or pour la vectorisation de principes actifs anticancéreux. Autre. Université de Limoges, 2016. Français. ⟨NNT : 2016LIMO0021⟩. ⟨tel-01334576⟩

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