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Metabolic Investigation of the Mycoplasmas from the Swine Respiratory Tract

Abstract : In this PhD thesis, we presented three main types of analyses of metabolism, and in most cases involving symbiosis: metabolic dialogue between a trypanosomatid and its symbiont, comparative analyses of metabolic networks and exploration of metabolomics data. The respiratory tract of swines is colonized by several pathogenic bacteria, among which are three mycoplasma species: Mycoplasma flocculare, Mycoplasma hyopneumoniae, and Mycoplasma hyorhinis. In this work, we created whole-genome metabolic network reconstructions for all sequenced strains from these three Mycoplasma species. Similar to other Mycoplasma models all reconstructed networks exhibit low connectivity due to the simplicity of the biological model. We were able to show that the three swine mycoplasma species have similar metabolic capabilities. Interesting metabolic differences include the myo-inositol catabolism and a more complete system for glycerol uptake in M. hyopneumoniae and a wide range of carbohydrate uptake in M. hyorhinis. Glycerol conversion to DHAP, a missing activity only in M. flocculare, produces toxic hydrogen peroxide and may explain the lack of pathogenicity of this species. The uptake of a wider range of carbon sources in M. hyorhinis may also explain why this species is a wide-known contaminant in cell cultures. Growth experiments showed that defined media described for other Mycoplasma species are not suitable for the growth of respiratory tract swine mycoplasmas and that peptone is essential for the maintenance of cell viability of both M. hyopneumoniae and M. flocculare in defined media. Metabolomic data suggests that even though these species are extremely similar from a genomic and metabolic point of views, the products and reaction rates differ and gene regulation may interfere directly in metabolism. This, in turn, may account for many aspects still unknown that influence directly different levels of pathogenicity in each of them
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Submitted on : Thursday, May 12, 2016 - 5:27:02 PM
Last modification on : Monday, February 10, 2020 - 4:36:55 PM
Long-term archiving on: : Tuesday, August 16, 2016 - 10:20:57 AM


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  • HAL Id : tel-01315159, version 1



Mariana Galvao Ferrarini. Metabolic Investigation of the Mycoplasmas from the Swine Respiratory Tract. Bioinformatics [q-bio.QM]. Université Claude Bernard - Lyon I, 2015. English. ⟨NNT : 2015LYO10302⟩. ⟨tel-01315159⟩



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