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Développement de méthodes de SRM à 4,7 T pour l'étude in vivo du métabolisme lipidique chez la souris.

Abstract : In recent years, there has been an unprecedented increase in the morbidity and mortality associated with diseases such as the steatosis, linked to obesity. In this context, pre-clinical and clinical studies are of interest in the search for new biomarkers allowing the diagnosis of steatosis. Currently, steatosis is diagnosed and graded by histological analyzes from a liver biopsy. On the other hand, it is advantageous to use non-invasive diagnostic modalities, especially in longitudinal studies. In this context, magnetic resonance spectroscopy (MRS), as a non-invasive and non-ionizing approach, is an attractive alternative method for the diagnosis of steatosis by measuring the hepatic fat fraction. Moreover, from the MRS spectrum acquired in the liver, it is possible to quantify the fatty acids (FA) composition of the hepatic lipids, which could be a potential biomarker for the follow-up of steatosis. The work of this thesis has been performed in vitro and in vivo, in the context of pre-clinical (4.7 T) and clinical (3.0 T) studies. An investigation of the optimal MRS acquisition protocol for the quantification of FA was carried out, with particular attention to the role of the water signal suppression module. Different quantification algorithms of the lipid composition were studied and validation of these algorithms was carried out in vitro and in vivo. Finally, still with the objective of determining new biomarkers of steatosis, a method (LOREEDE: LOngitudinal RElaxation time Evaluation from Dynamic Equilibrium) for the measurement in vivo of the T1 of the water resonance and the major lipid resonance, by MRS, was developped and validated in a preliminary study.
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Submitted on : Thursday, April 28, 2016 - 3:27:20 AM
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Amandine Coum. Développement de méthodes de SRM à 4,7 T pour l'étude in vivo du métabolisme lipidique chez la souris.. Médecine humaine et pathologie. Université Rennes 1, 2015. Français. ⟨NNT : 2015REN1B025⟩. ⟨tel-01308526⟩

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