Etude des mécanismes de formation des plaquettes sanguines : rôle de l'environnement médullaire

Abstract : Megakaryocytes differentiation (megakaryopoiesis) and platelet formation mechanisms are not entirely understood, but the bone marrow environment seems to be crucial in these processes. In this thesis, we show i) that integrin β3, the extracellular matrix protein receptors, are involved in megakaryopoiesis and platelet formation, ii) that recreating a 3D environment of stiffness in the range of that of bone marrow improves the maturation of in vitro differentiated megakaryocytes and iii) a new role for myosin IIA in the cytoplasmic distribution of organelles within the megakaryocyte. As a side-project, we characterized the specificity of expression of the Pf4-cre transgene to validate its use in our experimental approaches. This work enlightens new roles for myosin IIA and integrins in megakaryocytes and indicates that stiffness of the environment influences megakaryopoiesis.
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Fabien Pertuy. Etude des mécanismes de formation des plaquettes sanguines : rôle de l'environnement médullaire. Hématologie. Université de Strasbourg, 2014. Français. ⟨NNT : 2014STRAJ092⟩. ⟨tel-01294016⟩

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