Développement de PCRs multiplexes pour le diagnostic : microarrays analytiques

Stéphanie Cloux Boccoz 1
1 GEMBAS - Génie Enzymatique, Membrane Biomimétique et Assemblages Supramoléculaires
ICBMS - Institut de Chimie et Biochimie Moléculaires et Supramoléculaires
Abstract : The work reported in this thesis follows the one undertaken by Ms LE GOFF. It is focused on HIFI technology, which is patented and developed during her thesis. The first part of this work concerns the HIFI Blood 96™ test, and particularly the improvements and developments adduced to the test to make it a real diagnostic tool, multiparametric and high-throughput which can be implemented in blood banks in order to constitute negative antigen inventories, thus contributing to improve blood safety. It allows to characterize 96 samples on 15 polymorphisms (divided in two panels) associated to blood group systems in approximately 4.5 hours. A mesoscale validation study has been conducted on 583 samples for panel 1 and 190 samples for panel 2. The second part of this work describes the adaptation of HIFI technology applied to diagnosis of respiratory tract infections, with the development of another platform, ReSynPlex, in partnership with 3 research teams in Grenoble
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Stéphanie Cloux Boccoz. Développement de PCRs multiplexes pour le diagnostic : microarrays analytiques. Biotechnologies. Université Claude Bernard - Lyon I, 2015. Français. ⟨NNT : 2015LYO10282⟩. ⟨tel-01286987⟩

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