Développement de langue électronique : étude de mélanges complexes et de bactéries

Abstract : The objective of this PhD thesis is to explore the potential applications of the electronic tongue, based on combinatorial cross-reactive receptors and surface plasmon resonance imaging, for analysis and discrimination of different complex mixtures and bacteria. In this work, various complex mixtures of different nature such as wine, beer, and milk (either animal-based or plant-based) are used. It has been demonstrated that the electronic tongue is capable of responding differently to theses samples with good selectivity. For each of them, it can generate characteristic continuous 2D profile and 3D image, based on which the differentiation and classification of the complex mixtures have been carried out. Furthermore, it has been illustrated that the electronic tongue is efficient for monitoring the deterioration of milk. In the second part of this thesis, the electronic tongue has been applied for detection and analysis of bacteria. At first, some fluidic parameters have been optimized due to the variable morphology of these complexes and large biological objects. Under optimized experimental conditions, the electronic tongue is effective for analysis of bacteria with the possibility for quantification. Thereafter, the electronic tongue has allowed for the discrimination of different bacteria according to their genus, species and strains based on continuous 2D profiles and 3D images.
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Laurie-Amandine Garçon. Développement de langue électronique : étude de mélanges complexes et de bactéries. Matériaux. Université Grenoble Alpes, 2015. Français. ⟨NNT : 2015GREAI069⟩. ⟨tel-01258962⟩

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