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Ingénierie, photophysique et fonctionnalisation de chromophores pour la bio-photonique non linéaire in-vivo

Abstract : The use of two-photon absorbing (TPA) chromophore for applications in photodynamic therapy (PDT) and fluorescence imaging provides many advantages. The non-linear properties make it possible to increase both observation depth in animals and 3D resolution. Nevertheless, for in-vivo applications, improving bio-compatibility of these inherently lipophilic chromophore is a challenge.The development of new chromophores for PDT-TPA using a molecular engineering approach using bromide substituents as singlet oxygen generators is described. Parameters like position and number of bromide, the conjugated length and chromophore symmetry are studied. The study shows the importance of bromide atom position and of the symmetry on the inter system crossing efficiency. During the engineering study, spectroscopic observation and rationalization permit to envision the design of new chromophores for two photon laser scanning fluorescent microscopy. Bio-compatibility of these chromophores is provided by (hydroxyethyl)acrylate polymer, which provides a covalent water-soluble shell. These chromophores are used to make high resolution image of cerebral vascularization. One of these chromophores shows intravital specific interaction with endothelial cells in blood vessels. Some applications of the chromophore are described.Strategies to increase the intravital selectivity of polymer/chromophores units towards cancer cells and tumor are presented. A modification of hydroxyl function by imidazolium group is described. This new chromophore is evaluated towards its complexation properties with DNA and in cellulo spectroscopic studies.
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https://tel.archives-ouvertes.fr/tel-01247127
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Submitted on : Monday, January 4, 2016 - 3:04:20 PM
Last modification on : Thursday, November 21, 2019 - 2:32:31 AM
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  • HAL Id : tel-01247127, version 1

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Bastien Mettra. Ingénierie, photophysique et fonctionnalisation de chromophores pour la bio-photonique non linéaire in-vivo. Chimie organique. Ecole normale supérieure de lyon - ENS LYON, 2015. Français. ⟨NNT : 2015ENSL1038⟩. ⟨tel-01247127⟩

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