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Microbiocapteurs conductimétriques utilisant des oxydoréductases : de la conception à l’application à l’environnement et l’agroalimentaire

Abstract : Biosensors are rapid, selective and inexpensive devices that combine a biological recognition element, the so-called bioreceptor (e.g. enzymes, antibodies, DNA or microorganisms) to a physical transducer (e.g. electrochemical, optical, thermal or piezoelectrical). They can be used to detect one specific analyte or one family of analytes for a wide range of applications (e.g. environment, food, health). In this work, we developed biosensors based on three oxidoreductases (alcohol oxidase, lactate oxidase and formaldehyde dehydrogenase) for primary alcohols, lactate and formaldehyde analysis. The three enzymes were immobilized on interdigitated gold microelectrodes in view of conductometric detection. We tried to achieve a better understanding of biosensors processing and optimized enzyme immobilization as well as several fabrication or operational parameters to maximize analytical performances (selectivity, detection limit, reproducibility and stability). We also demonstrated the applicability of both conductometric biosensors based on lactate oxidase and formaldehyde dehydrogenase for the detection of lactate in dairy products and formaldehyde in waters
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Thanh Thuy Nguyen Boisse. Microbiocapteurs conductimétriques utilisant des oxydoréductases : de la conception à l’application à l’environnement et l’agroalimentaire. Chimie analytique. Université Claude Bernard - Lyon I, 2013. Français. ⟨NNT : 2013LYO10111⟩. ⟨tel-01175845⟩

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