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Extraction et caractérisation biochimique des polyphénol oxydases de champignons et leur application en biocatalyse supportée

Abstract : This work is devoted to the extraction of enzymes belonging to the polyphenol oxidase family from mushrooms, their biochemical characterization and their immobilization in solid hosts. These enzymes were first extracted from Paris mushrooms (Agaricus bisporus) and partially purified. A study of their enzymatic activity, stability conditions and thermal behavior was performed, together with the identification of inhibitors. A similar approach was applied to polyphenol oxidase extracted from desert truffle (Terfezia leonis Tul.). These enzymes were then trapped in silica gels for dopamine determination using an optical biosensor and in an alginate gel for phenol degradation.
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https://tel.archives-ouvertes.fr/tel-01158909
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  • HAL Id : tel-01158909, version 1

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Hicham Gouzi. Extraction et caractérisation biochimique des polyphénol oxydases de champignons et leur application en biocatalyse supportée. Chimie-Physique [physics.chem-ph]. Université Pierre et Marie Curie - Paris VI; Université Abou Bekr Belkaid (Tlemcen, Algérie), 2014. Français. ⟨NNT : 2014PA066668⟩. ⟨tel-01158909⟩

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