Synthèse de nanoparticules de dioxyde de titane de morphologies contrôlées : localisation, quantification et aspects toxicologiques de la cellule à l'organisme pluricellulaire

Abstract : Titanium dioxide nanoparticles are nowadays used in numerous domains. They enter in particular in the constitution of every day’s products such as paints or cosmetics (sun screen). The nanometer scale gives them a high reactivity which raises worries about their possible toxicity. Nanoparticles with controlled morphologies were synthesized and characterized. They were then introduced in the medium of human cells in cultivation in order to observe in vitro interactions and identify the reasons of this toxicity. The study was then extended to in vivo with a multi-cellular specimen: the nematode Caenorhabditis elegans (C. elegans). Researches have been focalized on detection, tracking and quantification of nanoparticles at the scale of single-cells and then in nematodes. The different kinds of synthesized nanoparticles allowed identifying the physico-chemical properties involved in their toxicity. Ion beam analyses were conducted in order to visualize and in some case quantify this nano-objects at the single cell-scale.
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Submitted on : Tuesday, February 24, 2015 - 3:43:14 PM
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Quentin Le Trequesser. Synthèse de nanoparticules de dioxyde de titane de morphologies contrôlées : localisation, quantification et aspects toxicologiques de la cellule à l'organisme pluricellulaire. Matériaux. Université de Bordeaux, 2014. Français. ⟨NNT : 2014BORD0047⟩. ⟨tel-01120012⟩

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