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Optimisation des conditions de migration et de détachement de lignées cancéreuses du cancer du sein en vue de leur tri fonctionnel

Abstract : This thesis investigates migration and detachement properties of different types of breast cancer cell lines : metastatic, invasive MDA-MB-231 and epithelial, low-invasive MCF-7 celles. Videomicroscopy and image analysis techniques were used to obtain dynamic information for large cell populations. Random migration assays performed on MDA-MB-231 cells reveal that their migration properties are related to both medium and surface (substrate adhesion protein type and quantity) conditions. A biphasic behavior for the migrated distance through time was observed to be dependent on the density of adsorbed protein (collagen type IV or fibronectin) on the substrate. Cell shape, detachement and moving properties were also computed as a function of the surface protein characteristics. These results were then used to perform directed migration assays in a chemotactic gradient generated in a microfluidic chamber. Optimal conditions for directed migration of cells were determined by varying the parameters of the system such as gradient maximum concentration, substrate adhesion protein… Lastly, it was experimentally proved that it is possible to separate cancer epithelial cell lines from mesenchymal ones by using chemotactic microfluidic chambers
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https://tel.archives-ouvertes.fr/tel-01119715
Contributor : Antoine Confavreux <>
Submitted on : Monday, February 23, 2015 - 6:23:41 PM
Last modification on : Thursday, October 15, 2020 - 8:54:02 AM
Long-term archiving on: : Wednesday, May 27, 2015 - 10:07:30 AM

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  • HAL Id : tel-01119715, version 1

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Antoine Confavreux. Optimisation des conditions de migration et de détachement de lignées cancéreuses du cancer du sein en vue de leur tri fonctionnel. Biophysique [physics.bio-ph]. Université Claude Bernard - Lyon I, 2014. Français. ⟨NNT : 2014LYO10209⟩. ⟨tel-01119715⟩

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