GFP amélioré). a) cette chimère localise les microtubules (b) la EGFP est attaché au N-terminus de la, pp.?-tubuline ,
Comparaison schématique des microscopies à champ large et confocale typiquement utilisées pour l'étude du vivant (source, p.17, 2006. ,
6 signaux de modulation Le contraste a été augmenté artificiellement pour améliorer la visualisation. Les molécules fluorescentes permettent de localiser le récepteur IFNAR1 marqué en GFP sur des cellules épithéliales (RPE1) ,
induit par le mouvement des endosomes sur les mesures de temps de vie de l'ordre de quelques nanosecondes (séquence 0.3), p.21 ,
Représentation de la variance calculée sur une mesure de référence FD FLIM de 12 images acquises avec un microscope confocal, p.23 ,
Gaussian Fitting 1992] est analogue à notre méthode, mais sans prise en compte de modèle d'intensité sinusoïdale. La méthode PPT est une méthode plus avancée combinant détection, puis calcul des trajectoires selon le principe du filtrage stochastique Les résultats sont obtenus sur des protéines RX marquées GFP et suivies manuellement (cellules épithéliales), p.26, 2006. ,
La fréquence d'acquisition est artificiellement réduite pour tester la robustesse de l'algorithme, p.36, 2011. ,
The thick horizontal lines denote electronic energy levels. The thinner grey lines represents the various vibrational energy states. Straight arrows are associated with absorption or emission of a photon. Wavy arrows illustrate molecular internal conversions or non-radiative relaxation processes. Vertical upward arrows indicate the instantaneous nature of excitation processes, p.48 ,
Total fluorescence intensity is shown in the center and corresponds to the sum of photon counts along the time axis at each pixel. The four side graphs correspond to time dependent photon counts in four dierent regions with variable sizes. By considering large regions, we observe an exponential fluorescence decay (see D) A: one pixel region; B and C: 3 ? 3 patches at dierent locations; D: 15 ? 15 patch and lifetime estimation by least mean squares fitting (commercial software), p.73 ,
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2006] and our method on fluorescently tagged RX protein in living epithelial cells. Endosomes have been tracked by hand using the MJtrack software, Mean localization error using Gaussian fitting Probabilistic particle tracking, p.111, 1992. ,
left) and membrane fluorescence lifetime map (right) in the presence of acceptor mCherry-KX 15 minutes after CX injection Among the KX-injected cells, only two cells present early endosomes that do not belong to the recycling compartment Additionally the lifetime estimated on the membrane exhibits strong variations, p.117 ,
A threshold for detecting large motion is estimated using the 99 Imaging with spinning disk confocal microscope Acquisition speed is set to 1 Hz for a minute Scale bar is 1 µm 192 10.13Evolution of large motion count with large motion threshold for control and treated cells. This is equivalent to a inverted cumulative histogram for the estimated velocities 193 10.14Error rate of u-track and iu-track wrt frame-rate decimation on a virus tracking experiment Frame-rate is artificially reduced to test the robustness of our tracker Frame-rate is artificially reduced to test the robustness of our tracker. On this example, we observe that u-track break point is around a 30-fold decimation while our improvement exhibits a single error at a 30-fold decimation (longer tracks on the last experiment are due to lower frame rate but represent real tracks) Imaging with spinning disk confocal microscope, 12A. Control cell. B. After nocodazole treatment 10.15Viruses tracking inside the cell, 0194. ,
Detection workflow as described in, 0201. ,
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