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Recherche de facteurs circulants impliqués dans la cardioprotection ischémique à distance par une approche protéomique

Abstract : Early reperfusion of occluded coronary artery significantly improves outcomes in patients with myocardial infarction. Unfortunately, this reperfusion can be the cause of irreversible myocardial damage, called reperfusion injuries. Remote ischemic preconditioning (RIPreC) is a simple and attractive strategy to reduce these myocardial injuries. Despite convincing evidence of the critical role played by circulating humoral mediators in RIPreC-induced cardioprotection, their actual identities remain unknown. Therefore, in this study we aimed to identify RIPreC-induced humoral mediators. In the first part of the study, we performed a proteomic analysis on rat plasma using SELDI-TOF-MStechnique. We thus related RIPreC to highly proteomic modulation. Overall, the proteins identified were inaccordance with a protective role of RIPreC. Some ofthem play a role in anti-oxidative stress, tissue repair, and anti-inflammatory process. In particular, apolipoprotein A-I (ApoA-I), significantly increased in rat plasma following RIPreC stimulus, has strong cytoprotective effects against ischemia-reperfusioninjury. It could then be a potential blood-borne factor of RIPreC. In the second part of the study we tried to demonstrate the cardioprotective properties of ApoA-I ina myocardial infarction model in vivo. ApoA-I injected before myocardial ischemia in rats significantly reduced myocardial infarct size. Taken together, our data indicated that RIPreC induces significant modifications of plasmatic proteome in vivo, notably ApoA-I increase.Furthermore, ApoA-I injection before myocardial ischemia-reperfusion was able to recapitulate RIPreC induced cardioprotection. ApoA-I may then be aprotective blood-borne factor involved in the RIPreC mechanism.
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https://tel.archives-ouvertes.fr/tel-01088630
Contributor : Anne-Marie Plé <>
Submitted on : Friday, November 28, 2014 - 12:02:03 PM
Last modification on : Monday, October 19, 2020 - 11:06:35 AM

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  • HAL Id : tel-01088630, version 1

Citation

Pierre Hibert. Recherche de facteurs circulants impliqués dans la cardioprotection ischémique à distance par une approche protéomique. Physiologie [q-bio.TO]. Université d'Angers, 2013. Français. ⟨tel-01088630⟩

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