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Adressage et immobilisation de biomolécules sur des surfaces : Interaction spot-dans-spot et bioélectrochimie localisée à l'échelle 1-50μm

Nathalie Berthet-Duroure 1
1 LAAS-NBS - Équipe NanoBioSystèmes
LAAS - Laboratoire d'analyse et d'architecture des systèmes
Abstract : The trend towards miniaturization of biochips and biosensors raises new issues. Indeed, the surfaces on which biomolecules must be addressed and grafted are getting smaller and smaller, and are also increasingly integrated into more and more complex systems. The Bioplume technology enables surface patterning at the 1-50μm scale. This promising approach was adapted to biomolecules deposition. The spot-in-spot technology was developed and proved to reduce biological samples expenses by 106 -109 times. Several grafting techniques were also carried out (GPTS silanisation, alcanethiols auto-assembly, PLLgPEG grafting and pyrrole electropolymerization), so that difficulties and performances were better clarified. Pyrrole electropolymerization was then adapted to the Bioplume system. This approach proved to be very efficient for depositing and grafting biomolecules on the surface in a single step. Thus, it was applied to the manufacture of single cell biochips and the functionalization of optical fibers. In the end, localised electrochemistry was further explored with three new approaches : PLLgPEG electrodesorption, droplet miniaturization using nanotips, and deposition onto dedicated microelectrodes. The major prospect is the industrial promotion of the Bioplume system. A new prototype will be developed to enable high-density protein chip manufacturing.
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  • HAL Id : tel-01071232, version 1

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Nathalie Berthet-Duroure. Adressage et immobilisation de biomolécules sur des surfaces : Interaction spot-dans-spot et bioélectrochimie localisée à l'échelle 1-50μm. Micro et nanotechnologies/Microélectronique. INSA de Toulouse, 2009. Français. ⟨tel-01071232⟩

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