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Functional characterization of GPI-anchored proteins of the SKU5/SKS gene family

Abstract : ABP1 (Auxin Binding Protein1), who can bind auxin, is essential for the development of plants. It was proved to have the ability to bind auxin and transduce auxin signal into the cells. It is supposed to be localized and functions at the outer surface of plasma membrane through unknown component. In my thesis, we tried to invesitgate the interaction between ABP1 and the candidate of the unknown component, CBP1 (From maize), which is GPI-acnhored and already identified as the binding ability to synthesized C-terminus peptide of ABP1 in 2006. The orthologous of CBP1 in arabidopsis belongs to a gene family with 19 members, in which only three of them were prediceted to be GPI anchored. We did the functional characterisation of these three GPI-anchored members. Data suggested that GPI-anchored SKS were involved in cell orientation, gametophyte and embryo development.
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Ke Zhou. Functional characterization of GPI-anchored proteins of the SKU5/SKS gene family. Plants genetics. Université Paris Sud - Paris XI, 2013. English. ⟨NNT : 2013PA112096⟩. ⟨tel-01066888⟩

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