Skip to Main content Skip to Navigation
Theses

Développement de méthodes bidimensionnelles en ligne LCxLC-MS pour l’analyse de composés chargés

Abstract : This manuscript is dedicated to the development of on-line two-dimensional liquid chromatography for the analysis of charged compounds with mass spectrometry coupling. The context of the study and the theoretical principles of liquid chromatography in one or two dimensions are presented. Experimental conditions such as instrumentation, columns, studied compounds are detailed. Some one-dimensional studies have been experimented to know the kinetic behaviour of small ionisables molecules and peptides depending on conditions. The orthogonality of chromatographic system and generated peak capacity have then been studied in order to know which experimental conditions are the most interesting for the analysis of charged compounds. The comparisons of systems have been done with new descriptor for orthogonality and effective peak capacity. Two-dimensional systems were then set up. Some limits in term of injected volume in the second dimension have been established in RPLC and in HILIC. The usefulness of a split to reduce the injected volume in second dimension has been studied. Some peptides mixtures have been separated by RPLCxRPLC(-MS), RPLCxHILIC(-MS) and HILICxRPLC. The MS coupling has been optimized. The interest of two dimensional separations is underlined compared to classical LC-MS separations
Document type :
Theses
Complete list of metadata

Cited literature [91 references]  Display  Hide  Download

https://tel.archives-ouvertes.fr/tel-01056279
Contributor : Abes Star :  Contact
Submitted on : Monday, August 18, 2014 - 2:22:11 PM
Last modification on : Tuesday, June 15, 2021 - 2:30:15 PM
Long-term archiving on: : Thursday, November 27, 2014 - 5:30:03 AM

File

TH2013_DAttoma-Dechenaux_Ameli...
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-01056279, version 1

Collections

Citation

Amélie d'Attoma. Développement de méthodes bidimensionnelles en ligne LCxLC-MS pour l’analyse de composés chargés. Chimie analytique. Université Claude Bernard - Lyon I, 2013. Français. ⟨NNT : 2013LYO10214⟩. ⟨tel-01056279⟩

Share

Metrics

Record views

579

Files downloads

3905