*. Tableau, *Récapitulatif*des*simulations*pour*les*designs*serpentins

!. Dans, ! gradient! et! dans! notre! cas! c'est! le! design!carré!qui!permet!la!plus!grande!force!magnétique.!Ce!design!sera!donc!privilégié!par!rapport! aux!autres

!. Les, !. , !. , !. , !. et al., !De!plus,!comme!nous!avons! pu!le!voir!dans!le!paragraphe!3.2.1,!chaque!matériau!possède!un!coefficient!d'extension!thermique! (CTE)! différent,! et! quand! la! résine! s'étend! en! se! réchauffant! et! en! se! rétractant

!. Avant, (Life!Technologies).!L'emploi!de!BSA!pour!recouvrir!les!parois!est!aussi! très! utilisé! en! microfluidique! mais! nous! gardons! cette! méthode! quand! nous! travaillons! avec! des! éléments!biologiques

!. Un, !. Dans-!-le, !. En, and !. Effet, !si!une!bulle!d'air!se!retrouve!dans!une!des!canalisations,!elle!modifie!grandement!la!résistance! fluidique! de! cette! dernière! modifiant! ainsi! l'équilibre! des! écoulements.! Malheureusement! nous! stabilisons!les!bulles!dans!nos!dispositifs!par!l'ajout!de!tensioactif.!De!plus!dans!le!design!3D,!la!partie! commune! offre! à! la! bulle! un! espace! plus! grand

!. Amine, 3! Sphérotec! Oui!(green)! Carboxyl! 5! Sphérotec! Oui!(green)! Carboxyl

*. Tableau, *Récapitulatif*des*billes*magnétiques*à*notre*disposition*avec*leurs*paramètres*respectifs

!. En and . Sphérotec, ! qui! selon! le! fabriquant! possède! «!une! forte! concentration! en! particules! ferromagnétiques!»

!. Nous and !. , design! de! première! génération.! Cette! différence! est! dûe! au! changement! des! billes! mais! pas! seulement

!. Nous, !. , !. , !. Bobines, and !. , non! alimentation! du! groupe! 3! rend! inopérante!l'étape!B!de!séparation.!Toutes!les!billes!sont!évacuées!dans!la!canalisation!supérieure,!et! cela

!. Une and !. Conducteur, ! Elle! constitue! une! inductance! mais! aussi! une! résistance! par! nature

!. Le, 8 m 2 !est! très! conséquent.! Or! nous! travaillons! dans! l'optique! de! manipuler! des! fluides! biologiques! avec! des! constituants!organiques!fragiles!tels!que!les!cellules.!Nous!devons!nous!assurer!que!l'augmentation! de!température!induite!par!l'effet!joule!ne!sera!pas!préjudiciable.! ! Pour!mesurer!l'échauffement, 2010.

!. Dans, notre!système!à! l'aide!d'un!module!Peltier.!La!rhodamine!est!conservée!dans!le!noir!afin!qu'elle!ne!se!détériore!pas! (bleaching)!à!la!lumière

!. Nous, !. , !. , !. , !. et al., ! ! Calibration!et!mesure!de!l'échauffement!:! ! Nous!avons!fait!varier!la!température!entre!22°C!(température!ambiante)!et!70°C

!. L-'intensité, !. , !. , !. , !. et al., ! Pour! les! différentes!températures!testées,!nous!obtenons!les!courbes!figures!150.! Nous!retrouvons!la!même!régression!linéaire!avec!un!coefficient!de!`0,01.!L'écart!des!deux!courbes! est! constant! illustrant! une! augmentation

!. Rèsuspendre, !. , !. , !. , and !. , (3` dimethylaminopropyl)!cabodiimide)! 4! Mélanger!à!température!ambiante

!. La, !. , !. , !. , !. et al., !Si!elles!ne!doivent!pas!servir!tout!de!suite,!les!billes!marquées! doivent!être!conservées!dans!un!milieu!de!stockage!particulier!constitué!de!PBS!avec!0

!. Les, !. , !. , and !. , !Juste!après!la!mise! en! solution! dans! l'eau! et! le! tween,! c'est`à`dire! après! déconditionnement! des! billes! de! leur! flacon! d'origine

!. Il, !. , !. , !. , !. et al., ! qui! d'ailleurs! ne! devrait! pas! jouer! sur! l'efficacité! de! marquage.! ! Nous! nous! sommes! rendu! compte! que! l'efficacité! de! marquage! était! très! variable

!. De, !. , !. , !. , !. et al., ! Cette! difficulté,!ne!doit!pas,!à!terme,!être!préjudiciable!car!l'étape!de!marquage!sera!réalisée

!. Etant, !. , !. , !. , !. et al., !nous!avons!souhaité!vérifier!l'expression! de!l'antigène!à!la!surface!des!monocytes!de!culture.!Pour!ce!faire,!nous!incubons!les!monocytes!avec! des!anticorps!fluorescents

*. Figure, *Test*fonctionnalisation*des*parois*des*monocytes*avec*des*anticorps*CD14

!. Dès, !que!ce!soit!juste!pour!une!observation!sur!lame! ou! pour! un! test! de! séparation! en! flux! dans! les! microcanaux,! il! s'est! révélé! difficile! d'observer! correctement! les! monocytes

!. Il, !. , !. , !. , !. et al., ! Il! existe! de! nombreuses! techniques! pour! marquer!une!cellule,!très!différentes!les!unes!des!autres.!Elles!se!caractérisent!par!un!marquage!de! cellules! vivantes! ou! mortes,! avec! une! facilité! plus! ou! moins! grande! pour! pénétrer! la! membrane,! intercalant! de! l'ADN?! Idéalement

!. La, rapport!F l h/?!R 3 !en!fonction!de!1`?!varie!de!façon!constante.! ! Cas!des!monocytes!:! ! Nous

*. Tableau, Paramètres*physiques*choisis*pour*un*monocyte

!. Concernant, !. , !. , !. , and !. 3d, analogie!avec!le!fonctionnement!pour!une!bille! seule!est!d'autant!plus!vraie!car!la!force!de!lift

!. Nous, !. , !. , !. , and !. Qu, une! seule! bille! était! fixée! sur! le! monocyte,! ce! qui! d'après! les! expériences! est! généralement! le! cas

!. Nous, !. , !. , !. , !. et al., !est!en!accord!avec!nos!expériences!réalisées! sur! les! billes! seules! et! avec! le! bilan! des! forces! précédemment! introduit, ! En! effet

*. Figure, *Piégeage*de*monocytes*colorés*à*l'Hoechst*et*marqués*avec*les*billes*magnétiques

!. Chapitre6, !. Manipulation, !. , and !. , !séparation!magnétique!de!monocytes! 172! ! sédimentation.! Mais! les! résultats! obtenus! n'en! sont! pas! moins! très! positifs

!. Ce, !. , !. , !. , !. et al., ! ! Nous!avons!montré!et!validé!le!procédé!de!fonctionnalisation!par!des!anticorps!CD14!de!nos!billes! magnétiques! pré`fonctionnalisé! NH2! de! 1µm! de! diamètre.! Ces! billes! fonctionnalisées! ont! pu! être! utilisées!pour!marquer,!en!tube!Ependorf

!. Le, !. , !. , !. , !. Loc-!-est-!-très-!-vaste et al., !une!fonction!que!nous!retrouvons!dans!de!nombreuses!applications!et!qui!fait!l'objet!de! beaucoup!de!recherches

!. La, !. , !. , !. , !. et al., ! Nous! avons! pu! démontrer! la! réalisation! d'écoulements! fluidiques!laminaires!3D!decòécoulements!superposés!sur!une!distance!contrôlée.!Ces!géométries! nous!ont!conduites!à!étendre!le!concept!du!T`sensor!et!à!une!application!d'étude!d'agrégation!d'une! protéine!l'Amyloïde!Béta

!. Une, !. De-!-base, !. , !. , !. et al., magnétiques,!puis!avec!des! monocytes!THP1!de!culture,!que!nous!avons!marqués!avec!des!billes!magnétiques.!Nous!avons!pu! démontrer! un! piégeage! et! une! séparation! maximale! dans! les! deux! cas! pour! des! conditions! d'écoulements!particulières

. Les&-approches&-hybrides&, canalisation,! le! tout! restant!commandable!afin!d'avoir!un!outil!précis!efficace!et!versatile.! ! Nous! avons! vu! les! limitations! d'une! telle! approche

!. Le, !. , !. , !. , !. et al., ! Dans! une! telle! situation! les! billes! magnétiques! ne! sont! soumises! à! aucune! force! et! leur! trajectoire! n'est!pas!modifiée.! ! Pour! créer! une! zone! de! champ! constant

!. Outre, augmentation!importante!du!champ,!l'utilisation!d'aimants!permanents!permet

*. Figure, * Preuve* du* concept* d'attraction* et* de* répulsion* grâce* à* l'ajout* d'aimants* permanents* en* plus* des* microbobines

!. Nous and !. , !de!plus!la!zone! de!travail!est!plutôt!petite!ce!qui!impose!un!positionnement!précis!de!la!puce!entre!les!aimants.! Les&avantages&d'ajout&d'aimants&permanents&pour&notre&étude& ! Nous!avons

!. Il, !. , !. Qu-'en-!-changeant-!-de-!-géométrie, and !. ?. , aimants.! Cependant!là!où!nous!perdons!un!peu!de!«!puissance!»!magnétique!nous!gagnons!énormément!en! réalisation!technique.!En!effet

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!. Il, générées!par!l'écoulement.!Nous!pourrions!dire!que!la!séparation!et!la!concentration!de!l'échantillon! se! fait! «!naturellement!»! à! condition! d'avoir! la! bonne! géométrie! de! puce

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