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Dévelopement d'outils microfluidiques appliqués à la biologie. Réalisation de dispositifs de tri cellulaire magnétique vertical.

Sébastien Cargou 1 
1 LAAS-N2IS - Équipe Nano Ingénierie et Intégration des Systèmes
LAAS - Laboratoire d'analyse et d'architecture des systèmes
Abstract : Thanks to advances in research over the last ten years, the implementation of more and more functions in a microfluidic environment, "lab-on-chip" has become a reality especially in the point of care field. First devices are emerging on the commercial market, but it remains a major challenge, the detection of elements in low concentrations that circulate in the blood for example. Separation techniques which are simple, efficient and repeatable are the key to most of those problems. We present in this work, an original way of magnetic separation using planar copper coils combined with a 3D microfluidic system for efficient vertical separation. The study involves the design, the modeling, the realization and testing of devices first with solutions made with magnetic beads then with organic compounds from culture. The lab-on-chip are made by photolithography process and thanks to an original lamination technology of dry film. Due to its great robustness in time and face solvents, as well as its biocompatibility, SU8 is a prime candidate for the realization of these LOC. This technology allows us to realize tridimensional all polymer chips with active functions like magnetic actuation. Microelectronics has for a long time miniaturized all electronic components such sensors and coils. It is then very interesting to see how we can integrate them into a microfluidic device to create more complex functions. Now, we are able to have a continuous separation of culture THP1 monocytes with an efficiency of 82% and routes promising improvements validated with magnetic beads
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Submitted on : Tuesday, May 27, 2014 - 10:51:33 AM
Last modification on : Monday, July 4, 2022 - 8:48:00 AM
Long-term archiving on: : Wednesday, August 27, 2014 - 11:10:13 AM


  • HAL Id : tel-00996924, version 1


Sébastien Cargou. Dévelopement d'outils microfluidiques appliqués à la biologie. Réalisation de dispositifs de tri cellulaire magnétique vertical.. Micro et nanotechnologies/Microélectronique. Université Paul Sabatier - Toulouse III, 2014. Français. ⟨tel-00996924⟩



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