Développement de méthodes de spectroscopie par résonance magnétique localisée pour l’étude du métabolisme chez le petit animal

Abstract : Here we present a 1H −13 C filtered method for localized spectroscopy for the study of mice brain metabolism and, also for the development of localized HRMAS spectroscopy methods for the in vivo study of drosophila. The aim of the work on mice was to study the effect of a vascular disrupting agent on glioma. We had to build a probe (cradle + detector) dedicated to this work. With this work we succeed to show the contribution of the 1H −13 C spectroscopic method, compared to the classical one, 1H spectroscopy. For the drosophila part of my work we succeed to perform slice selective spectroscopy on living drosophila using high Resolution Magic Angle Spinning, we even perform chemical shift imaging (1 spatial dimension) which give us a mapping of the metabolites distribution. As far we known, those methods represent two « premieres », and offer new perspectives of research on this insect, which is the most used in this field, particularly because of the numerous genetic studies on this animal.
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Nicolas Joudiou. Développement de méthodes de spectroscopie par résonance magnétique localisée pour l’étude du métabolisme chez le petit animal. Autre [cond-mat.other]. Université d'Orléans, 2013. Français. ⟨NNT : 2013ORLE2044⟩. ⟨tel-00994601⟩

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