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Identification et validation fonctionnelle de gènes candidats contrôlant la composition de la cuticule chez le fruit de tomate

Abstract : The cuticle, an extracellular lipid matrix consisting of waxes and of a cutin skeleton is the outermost plants protection barrier against their environment. The cuticle is involved in many agronomic traits such as post-harvest storage, biomechanical or fruit appearance properties like surface brightness. In order to isolate cuticle mutants, the screening of an EMS tomato mutants collection has been undertaken, based on fruit brightness, leading to the selection of 24 mutants. Biochemical analyzes have shown wide variations in cuticle loads and compositions, especially in cutin mutants. The characterization of 4 remarkable mutants was undertaken to identify the mutations responsible for brightness phenotypes. The most affected mutant shows a cutin load reduced by 85% compared to the wild type, and is due to a mutation in the SlGDSL2 gene, encoding a GDSL-motive acylhydrolase enzyme, responsible for the cutin polymerization. In order to further study the cutin formation and regulation, the next work was to obtain and characterize single and double mutants affected in cutin monomer synthesis, apoplastic transport and cutin polymerization.
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Submitted on : Thursday, March 27, 2014 - 4:42:08 PM
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PETIT_JOAHNN_2013.pdf
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  • HAL Id : tel-00966997, version 1

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Johann Petit. Identification et validation fonctionnelle de gènes candidats contrôlant la composition de la cuticule chez le fruit de tomate. Sciences agricoles. Université Sciences et Technologies - Bordeaux I, 2013. Français. ⟨NNT : 2013BOR15225⟩. ⟨tel-00966997⟩

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