Diversité génétique et recherche de facteurs de virulence de Nosema ceranae, parasite de l'abeille mellifère

Abstract : The microsporidian parasite Nosema ceranae is an emergent pathogen of the Western honeybee (Apis mellifera). It is associated to a disease called nosemosis that can lead to high mortality of honybees in colonies. Its presence in hives has not been systematically linked to symptoms or depopulation, suggesting a variation in its virulence. Thus, the existence of several N. ceranae variants with different virulence levels has been proposed. In this work aimed to assess N. ceranae polymorphism through a multilocus approach to test whether is it possible to discriminate between parasite taxa. Thus the nucleotide diversity of ten marker genes has been measured in parasite populations isolated from single A. mellifera individuals in distant locations. While high nucleotide diversity and allele content have been observed for all genes in single individuals, the absence of isolate differentiation precluded any taxa discrimination. These data support the hypothesis of a recent host-jump to A. mellifera and suggest that similar populations of parasites infect honeybees in distant locations. However they question the origin of such polymorphism within one host. In the second part of this work genes encoding putative virulence factors have been searched within N. ceranae genome, in order to produce recombinant proteins and then specific antibodies. Such antibodies would allow the subcellular localization of those proteins in infected tissues.
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Mathieu Roudel. Diversité génétique et recherche de facteurs de virulence de Nosema ceranae, parasite de l'abeille mellifère. Sciences agricoles. Université Blaise Pascal - Clermont-Ferrand II, 2013. Français. ⟨NNT : 2013CLF22414⟩. ⟨tel-00959521⟩

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