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Les liposomes biphényles : un nouveau modèle de biomembrane magnétique fluorescent : caractérisation par RMN des solides, microscopies optiques et électroniques et SAXS

Abstract : A new model of biomembrane (liposome) was developed from synthesized lipids containing a biphenyl unit on the sn2 aliphatic chain and possessing a sn1 aliphatic chain which varies in length and unsaturation. The positive magnetic susceptibility anisotropy of these molecules induces an oblate deformation of these «biphenyls » liposomes under the magnetic field B0. This particular deformation has been characterized by 31P and 2H solid state NMR by varying different parameters: the intensity of B0, the membrane elasticity, the temperature and the size of the liposomes (Helfrich, 1973). These deformed vesicles were observed by optical and electron microscopy and the remanence of the deformation outside B0 has been analyzed by Small angles X-ray scattering (SAXS).Finally, the first applications of biphenyls liposomes as new biomembrane model to analyze the structure and orientation of membrane proteins or peptides were studied by 15N solid state NMR
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Nicole Harmouche. Les liposomes biphényles : un nouveau modèle de biomembrane magnétique fluorescent : caractérisation par RMN des solides, microscopies optiques et électroniques et SAXS. Autre. Université Sciences et Technologies - Bordeaux I, 2013. Français. ⟨NNT : 2013BOR15213⟩. ⟨tel-00959353⟩

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