Skip to Main content Skip to Navigation
Theses

Evaluation thermodynamique et biologique d’un substituant synthétique aux produits d’origine animale dans les solutions de cryoconservation pour embryons de mammifères

Abstract : Several compounds in embryo cryopreservation solutions are a source of concern:products of animal origin because of the sanitary risks, and the permeating cryoprotectantsbecause of their potential mutagenic effect. Removing or substituting these compounds withchemically defined products might improve embryo cryopreservation technics.Conception and use of cryopreservation protocols are often empirical. This empiricismleads to many variations between the studies which make a comparison between results allthe more difficult. In our study, two complementary approaches were associated:• The first approach (physical) consisted of using the differential scanning calorimetry tostandardize the comparison between different slow-freezing solutions. So, thethermodynamic properties of solutions containing a potential substitute were characterizedand compared to those obtained with solutions containing reference products (fetal calfserum and bovine serum albumin) ;• The second approach (biological) consisted of using freezing of in vivo-produced rabbitembryos or freezing of in vitro-produced bovine embryos in order to evaluate survival
Document type :
Theses
Complete list of metadatas

Cited literature [132 references]  Display  Hide  Download

https://tel.archives-ouvertes.fr/tel-00936111
Contributor : Abes Star :  Contact
Submitted on : Friday, January 24, 2014 - 3:07:08 PM
Last modification on : Tuesday, February 11, 2020 - 1:43:18 AM
Long-term archiving on: : Thursday, April 24, 2014 - 10:56:30 PM

File

TH2012BruyerePierre.pdf
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-00936111, version 1

Collections

Citation

Pierre Bruyère. Evaluation thermodynamique et biologique d’un substituant synthétique aux produits d’origine animale dans les solutions de cryoconservation pour embryons de mammifères. Sciences agricoles. Université Claude Bernard - Lyon I, 2012. Français. ⟨NNT : 2012LYO10145⟩. ⟨tel-00936111⟩

Share

Metrics

Record views

274

Files downloads

1601