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Synthèse de complexes luminescents à base d'ions lanthanides pour le marquage de biomolécules : application au diagnostic de la maladie d'Alzheimer

Abstract : This work aimed at the design of markers with lanthanides ions for the early diagnosis of the Alzheimer's disease, through an energy transfer with luminescent quantum dots. Our choice focused on polyaminophosphonated ligands bearing a pyridine-bis-pyrazolyl moiety and made reactive towards biomolecules by the presence of activated groups. We present in the synthesis of a model ligand incorporating only the tridentate photosenstization unit and phosphonate moieties. From this ligand, europium and terbium complexes have been synthesized and characterized. An important stability and advantageous luminescence properties were revealed by our studies, especially for the terbium complex.Then a method of synthesis of ligands of this type, functionalized by the presence of reactive groups was developed and labeling experiments were carried aout with several biomolecules amoung which some Alzheimer’s disease biomarkers.The synthesis of another ligand linked covalently to biotin and the use of the corresponding terbium complex allow the study of FRET with various luminescent energy acceptors among which some quantum dots.
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Katia Nchimi Nono. Synthèse de complexes luminescents à base d'ions lanthanides pour le marquage de biomolécules : application au diagnostic de la maladie d'Alzheimer. Autre. Université de Strasbourg, 2012. Français. ⟨NNT : 2012STRAF045⟩. ⟨tel-00923166⟩

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