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Comparaison de procédés d'extraction appliqués au domaine des biotechnologies blanches

Abstract : This aim of this study was to compare liquid-liquid extraction (LLE) and extraction by emulsion (ELM) of biomolecules as gallic acid. TBP as the extractant and Na2HPO4 as the stripping agent were chosen thanks to their efficiency. The influence of many parameters on these two processes was studied to determine their mechanism. LLE process is limited by equilibrium: the extraction is efficient for high TBP concentrations (> 20 % v/v). This is not the case for ELM process: if the emulsion was stable, the extraction was quantitative within a few minutes with lower extractant concentrations, which made ELM process more environment-friendly. By using agrodiluents to replace dodecane for more environmental-friendly processes, interesting results were obtained. In ELL, higher extraction yields were obtained with TBP diluted in ethylic esters of fatty acids. Even, in ELM, gallic acid was quantitatively extracted without TBP.
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Submitted on : Wednesday, November 27, 2013 - 12:18:11 PM
Last modification on : Wednesday, July 1, 2020 - 4:30:04 PM
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  • HAL Id : tel-00910070, version 1



Ka Ho Yim. Comparaison de procédés d'extraction appliqués au domaine des biotechnologies blanches. Autre. Ecole Centrale Paris, 2013. Français. ⟨NNT : 2013ECAP0015⟩. ⟨tel-00910070⟩



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