Skip to Main content Skip to Navigation
Theses

Etude dynamique et structurale de biomolécules par microscopie à force atomique HS-AFM : application à une petite protéine de choc thermique sHsp

Abstract : The atomic force microscopy (AFM) gives access to the topography of organic and inorganic samplesat the atomic scale. The latest innovations offer the possiblity to understand the sample nano-mechanicalproperties (elasticity, adhesion...). Its feature set allows overcoming the demands of nanotechnology,both in the fields of physics, chemistry and biology.However, understanding biological processes require faster acquisitions for the atomic forcemicroscopy, less than a second per frame. As conventional equipment does not offer the possibility toovercome the constraint of time for dynamical studies, a prototype of high-speed atomic forcemicroscope (HS-AFM) was developed in partnership with Professor T. Ando group of Kanazawa University(Japan). It can reach scanning video speed: 25-50 frames/s in a liquid medium. The device is beingconstantly improved: new feedback control, larger scanning sizes. The resolution is provided byminiaturized cantilevers with carbon EBD-tips. In parallel to innovative modules on the microscope, addonshave been developed: syringe pump and heating modules.The potential of the prototype, developed within the framework of the program ANR PNANO 2008HS-nanobio-Imaging, has been shown through the study of a small heat shock protein: the protein sHspLo18. This protein, from the lactic acid bacterium Oenococcus oeni, offered the possibility of a variouschanges of oligomerization degrees according to the pH, and also the chaperone and lipochaperon activityof protein under the influence of an environmental stress. The use of these techniques of microscopiescoupled with biochemical studies on this proteic model allowed to dread the effect of surfaces on theadsorption and the dynamics of biological complexes. The interaction protein – surface coulb be toapprehend and proves to be useful for the development of protein sensors developed in the laboratory
Complete list of metadata

Cited literature [167 references]  Display  Hide  Download

https://tel.archives-ouvertes.fr/tel-00873331
Contributor : Abes Star :  Contact
Submitted on : Tuesday, October 15, 2013 - 2:53:09 PM
Last modification on : Friday, June 8, 2018 - 2:50:14 PM
Long-term archiving on: : Thursday, January 16, 2014 - 7:35:17 AM

File

these_A_CARRIOU_David_2012.pdf
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-00873331, version 1

Collections

Citation

David Carriou. Etude dynamique et structurale de biomolécules par microscopie à force atomique HS-AFM : application à une petite protéine de choc thermique sHsp. Autre [cond-mat.other]. Université de Bourgogne, 2012. Français. ⟨NNT : 2012DIJOS082⟩. ⟨tel-00873331⟩

Share

Metrics

Record views

1033

Files downloads

1477