Skip to Main content Skip to Navigation
Theses

Sensibilité du coeur à l’ischémie-reperfusion et stratégie de cardioprotection par l’exercice : rôle spécifique de la eNOS myocardique

Abstract : Exercise training is recognized as an efficient way to protect the myocardium against ischemiareperfusion(IR). However, mechanisms responsible for such cardioprotection remain still unclear. Theaims of this work were then i) to evaluate the preventive effect of exercise on a model highly sensitiveto myocardial IR, and ii) to investigate the role of eNOS in exercise-induced cardioprotection. In a firstpart we showed that regular boots of exercise, by its beneficial effects on calcium handling andenzymatic antioxidant status, prevents the highly sensitive phenotypical remodeling of the heart andthen normalized heart vulnerability to IR. Then, in a second part of this work, we showed that exerciseinducedcardioprotection was associated with a decrease of eNOS phosphorylation at Ser1177 andespecially its uncoupling during early reperfusion. Such phenomenon, associated with increased heartantioxidant capacity was responsible for reduced nitro-oxidative stress. Indeed, reduced NOSdependentNO synthesis associated with the improved capacity to scavenge O2.- contribute to preventthe formation of ONOO-. Altogether, these results showed that exercise-induced cardioprotection is acomplex mechanism requiring interactions between antioxidant capacity improvement, eNOSuncoupling during reperfusion and intracellular calcium homeostasis. Finally, this work opens newperspectives regarding the role of NO synthesis modulation to impact heart sensitivity to IR
Document type :
Theses
Complete list of metadatas

Cited literature [182 references]  Display  Hide  Download

https://tel.archives-ouvertes.fr/tel-00870574
Contributor : Abes Star :  Contact
Submitted on : Monday, October 7, 2013 - 3:47:13 PM
Last modification on : Thursday, August 13, 2020 - 3:00:48 AM

File

pdf2star-1381141157-these_fara...
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-00870574, version 1

Collections

Citation

Charlotte Farah. Sensibilité du coeur à l’ischémie-reperfusion et stratégie de cardioprotection par l’exercice : rôle spécifique de la eNOS myocardique. Sciences agricoles. Université d'Avignon, 2012. Français. ⟨NNT : 2012AVIG0706⟩. ⟨tel-00870574⟩

Share

Metrics

Record views

691

Files downloads

4419