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Caractérisation fonctionnelle de l'activité de l'histone acétyltransférase GCN5 au sein des complexes ATAC et SAGA chez l'homme

Abstract : In order to initiate the transcription by the RNA polymerase II, chromatin needs to be modified by coactivators. Some of these coactivators are histone post-translational modifying complexes. GCN5 is a histone acetyltransferase enzyme (HAT), which can acetylate the histones. This enzyme is found in a multiproteic complex named SAGA. Recently, a second HAT complex containing GCN5 was discovered: ATAC, in drosophila. At the beginning of my thesis, the existence of such complex in human was not shown.My thesis objectives were then to identify and characterize an ATAC complex in human cells. In a first part, we purified and identified the composition in subunits of human ATAC. Then we studied the activity and specificity of ATAC on histone substrates, compared to SAGA. Next, we were wondering how the subunits of the two HAT complexes could play a role on the regulation of the activity of the enzyme GCN5, in order to understand the histone specificity of ATAC and SAGA.
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Anne Riss. Caractérisation fonctionnelle de l'activité de l'histone acétyltransférase GCN5 au sein des complexes ATAC et SAGA chez l'homme. Biochimie, Biologie Moléculaire. Université de Strasbourg, 2012. Français. ⟨NNT : 2012STRAJ048⟩. ⟨tel-00868785⟩

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