Skip to Main content Skip to Navigation
Theses

Altérations fonctionnelles et phénotypiques des cellules dendritiques plasmacytoïdes et des lymphocytes T régulateurs dans le cancer de l’ovaire

Abstract : Ovarian cancer (OC) is an immunogenic disease and represents a good model for studying antitumoral immunity. We performed a systematic comparison between plasmacytoid dendritic cells (pDC) and regulatory T cells (Treg) in blood, ascites, and tumors in term of frequencies, phenotypes, functions, and impact on outcome of OC patients. We found that pDC accumulate in ascites and are present in some tumors whereas they are profoundly depleted in patients’ blood. Their presence within tumors (but not ascites) is deleterious because associated with early relapse of OC patients. Moreover, Tumor associated pDC (TApDC) but not ascite pDC were altered in their innate function, i.e. the production of IFN-α in response to TLR ligands in vitro, and they induce the development of IL-10+ CD4+T cells. All these results suggest that TApDC but not ascite pDC induce immune tolerance allowing cancer progression. Treg accumulate in ascites and tumors but their levels in patients’ blood were not increased. Their accumulation in tumors, but not ascites, was an independent prognostic factor associated with delayed relapse. TATreg showed an activated phenotype and inhibit IL-10 production by CD4+conventional TAT cells. Interestingly, patients whose tumor infiltration by Foxp3+ Treg is increased after neoadjuvant chemotherapy showed delayed relapse suggesting that chemotherapy, in addition to its direct antitumoral effect, induces an immune response
Document type :
Theses
Complete list of metadatas

Cited literature [177 references]  Display  Hide  Download

https://tel.archives-ouvertes.fr/tel-00861852
Contributor : Abes Star :  Contact
Submitted on : Friday, September 13, 2013 - 4:18:44 PM
Last modification on : Friday, October 23, 2020 - 5:03:34 PM
Long-term archiving on: : Thursday, April 6, 2017 - 7:40:19 PM

File

TH2011_Labidi-Galy_Sana-Intidh...
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-00861852, version 1

Collections

Citation

Sana Intidhar Labidi-Galy. Altérations fonctionnelles et phénotypiques des cellules dendritiques plasmacytoïdes et des lymphocytes T régulateurs dans le cancer de l’ovaire. Médecine humaine et pathologie. Université Claude Bernard - Lyon I, 2011. Français. ⟨NNT : 2011LYO10183⟩. ⟨tel-00861852⟩

Share

Metrics

Record views

804

Files downloads

1398