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Intérêts et limites de l'analyse de la moelle osseuse en toxicologie médicolégale : contribution à l'interprétation quantitative des concentrations médullaires

Abstract : The aim of this work was to evaluate the interest of bone marrow (BM) analysis in forensic toxicology, as an alternative matrix to blood. An analytical method was developed and validated for the quantification of citalopram, diazepam, and its main metabolites (nordazepam, temazepam, oxazepam) in BM and 10 others matrices of forensic interest. This procedure was successfully applied to real cases for putrified sample analyses and to establish a tissue kinetic in rabbit samples for a pharmacokinetic study. These animals kinetics were implemented in PBPK modeling to predict in human tissue distribution of citalopram, diazepam, and its metabolite, nordazepam, after oral therapeutic administration. These predictions gave some clues to interpret quantitatively tissue concentrations in forensic toxicology. A study was also performed to examine whether BM sample location may influence post mortem BM quantification and correlation between BM and blood concentrations. Caffeine was used as test compound. Sample location was found to be an important parameter to consider in quantitative interpretation of BM analyses. This work confirmed the interest of BM in forensic toxicology. Experimental studies improved our knowledge on this matrix about the problematic of sample location, analytical procedure and ante mortem distribution to contribute to qualitative and quantitative interpretation of BM analyses
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Nathalie Cartiser. Intérêts et limites de l'analyse de la moelle osseuse en toxicologie médicolégale : contribution à l'interprétation quantitative des concentrations médullaires. Médecine humaine et pathologie. Université Claude Bernard - Lyon I, 2011. Français. ⟨NNT : 2011LYO10149⟩. ⟨tel-00847114⟩

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