Déterminants protéiques de la voie de sécrétion Sec impliqués dans la formation de biofilm chez Listeria monocytogenes

Abstract : Listeria monocytogenes is a foodborne pathogenic bacteria responsible for listeriosis, a rare but high mortality rate disease in humans (25 %). This bacterium can form biofilm allowing a better resistance to environmental stresses as well as decontamination treatments. A new strategy for genomic analysis was developed and allowed to target secretion systems and proteins potentially involved in biofilm formation. Inactivation of the SecA2 pathway leads to the formation of an aerial and fragile biofilm. This morphotype is able to grow in a sessile mode at 20 °C on polystyrene whereas this is not the case for the wild type strain. New proteins secreted in a SecA2 manner were identified by comparing the ΔsecA2 exoproteome to the one of the wild type. The role of lipoproteins in biofilm formation and their maturation by the signal peptidase II, LpsA and LspB, was also tackled. Combining expression analysis of genes encoding lipoproteins during biofilm formation with genomic analysis based on the secretome allowed targeting three lipoproteins, including LpeA, which appeared to be involved in the later stages of biofilm formation. Finally, the importance of LspA in the maturation of lipoproteins,was highlighted by comparing of the double mutant ΔlgtΔlspA and ΔlgtΔlspB exoproteomes to the one of Δlgt.
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Sandra Anne Angèle Renier. Déterminants protéiques de la voie de sécrétion Sec impliqués dans la formation de biofilm chez Listeria monocytogenes. Sciences agricoles. Université Blaise Pascal - Clermont-Ferrand II, 2012. Français. ⟨NNT : 2012CLF22307⟩. ⟨tel-00844396⟩

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