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Caractérisation structurale et perception par la plante hôte Medicago truncatula des chitosaccharides pariétaux d'Aphanomyces euteiches, parasite de légumineuses

Amaury Nars 1
1 Interactions Plantes-Microorganismes
LRSV - Laboratoire de Recherche en Sciences Végétales
Abstract : The oomycete Aphanomyces euteiches is a root pathogen infecting legumes which causes important yield losses. The cell wall of A.euteiches contains 10% N-acetylglucosamine (NAG) in the form of non-crystalline chitosaccharides, which are associated to cell wall glucans. To study their biological activity, a root elicitation bioassay on the host plant Medicago truncatula has been set up using a preparation of chitin fragments as a control elicitor. The purification of hydrolysed cell wall fractions enriched in NAG yielded glycan fragments which were composed of glucose and NAG. These heteropolymers possess a novel structure, which has never been described before. The root elicitation bioassay showed a biological activity of the cell wall fractions different from the one of chitin fragments, on M.truncatula. Interestingly, one fraction induced nuclear calcium oscillations in epidermal cells of M.truncatula root cultures, which are different from the response evoked by pure chitotetramers.
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https://tel.archives-ouvertes.fr/tel-00843419
Contributor : Amaury Nars <>
Submitted on : Thursday, July 11, 2013 - 12:21:27 PM
Last modification on : Friday, January 10, 2020 - 9:10:17 PM
Long-term archiving on: : Wednesday, April 5, 2017 - 9:34:14 AM

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  • HAL Id : tel-00843419, version 1

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Amaury Nars. Caractérisation structurale et perception par la plante hôte Medicago truncatula des chitosaccharides pariétaux d'Aphanomyces euteiches, parasite de légumineuses. Biologie végétale. Université Paul Sabatier - Toulouse III, 2013. Français. ⟨tel-00843419⟩

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