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Suivi 3D de nanoparticules d'or par holographie digitale

Abstract : We present a new technique that combines off-axis Digital Holography and Dark Field Microscopy to track in three dimensions gold nanoparticles diffusing in a fluid or in biological medium. We show that a single hologram is sufficient to localize several particles in a thick sample with a localization accuracy independent of the particle position. First, we remind the principle of digital holography then we present existing technique of 3D tracking using holography. We explain why we use a dark field illumination because of the small scattering cross section of nanoparticles. Then we describe the optical setup and the software developed to reconstruct holograms in real time. Finally, we present experimental results of 3D tracking of brownian nanoparticles and preliminary results of localization in living cells.
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Submitted on : Friday, June 21, 2013 - 5:32:57 PM
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  • HAL Id : tel-00836925, version 1


Frédéric Verpillat. Suivi 3D de nanoparticules d'or par holographie digitale. Instrumentations et Détecteurs [physics.ins-det]. Université Pierre et Marie Curie - Paris VI, 2012. Français. ⟨NNT : 2012PAO66301⟩. ⟨tel-00836925⟩



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