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Theses

Création de surfaces antibiofilms par greffage covalent de peptides et enzymes antibactériens sur les surfaces de cuivre et de titane

Abstract : Bacterial contamination and formation of biofilms on immersed structures in seawater are frequent and at the origin of high cost maintenance treatments. The use of compounds biocidal, but also harmful for the ecosystem, is nowadays forbidden. The aim of this thesis work was to find new preventive surface treatments to stop, or strongly reduce, biofilm formation on copper and titanium surfaces. This is possible by preventing bacteria adhesion and, if necessary, their development after their adhesion. Copper surfaces were functionalized by crosslinkers onto which two antimicrobial molecules, Magainin I and HEWL lysozyme, were then grafted. An alternative strategy consisted in grafting spacers, and antimicrobial agents, on a copper surface microstructured by using a photopolymer derived from poly(methyl methacrylate). Magainin I and HEWL were also grafted onto titanium surfaces via two kinds of spacers, a silane and a diamino-PEG. Antibacterial surface treatments were optimized after characterization of each step by IR, XPS, AFM and angle contact measurements. In order to highlight the antibiofilm properties of functionalized surfaces, four tests were conducted: one test for protein adsorption, one measuring bacteria adhesion and one testing their viability; the last one was conducted to assay bacteria growth after deposition onto functionalized surfaces. As examples, bacteria growth was reduced by 90% and 80 % onto functionalized titanium and copper surfaces respectively
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  • HAL Id : tel-00833434, version 1

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Jessie Peyre. Création de surfaces antibiofilms par greffage covalent de peptides et enzymes antibactériens sur les surfaces de cuivre et de titane. Matériaux. Université Pierre et Marie Curie - Paris VI, 2012. Français. ⟨NNT : 2012PAO66535⟩. ⟨tel-00833434⟩

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