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Perméabilisation photocontrôlée de la membrane biologique : étude en systèmes modèles et en cellules

Abstract : Lipid membrane permeabilization is nowadays an important area of research, since the transport of small molecules (like DNA, proteins, etc) into the cells still needs to be improved. In this work, we try to contribute to this study by proposing a non-invasive and easily controlled way of permeabilization by the use of Azobenzene Modified Polymers (AMP). Copolymers with balanced amphiphilicity have been previously shown to permeabilize lipid membranes. The AMP would permit us to control the permeabilization of the membrane by controlling their hydrophobicity via the radiation exposure wavelength. This hypothesis has been verified using Giant Unilamellar Vesicles (GUV) containing soluble fluorescent probes. The kinetics of the leakage of these probes in combination with electrophysiology experiments in Black Lipid Membranes (BLM) give us evidence to go further on the characterization of the permeabilization structures created by the lipid-polymer interactions. In addition, experiments have been performed on Chinese Hamster Overy cells (CHO). The possibility of using AMP for the delivery of molecules not able to cross the membrane by themselves has been examined and the release of molecules "trapped" in the cells and its kinetics have been studied
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  • HAL Id : tel-00833272, version 1

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Dimitra Milioni. Perméabilisation photocontrôlée de la membrane biologique : étude en systèmes modèles et en cellules. Biophysique. Université Pierre et Marie Curie - Paris VI, 2012. Français. ⟨NNT : 2012PAO66528⟩. ⟨tel-00833272⟩

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