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Développements méthodologiques en spectrométrie de masse et en mobilité ionique pour l'étude d'assemblages supramoléculaires en biologie

Abstract : The aim of this thesis was the development of different supramolecular approaches, like MS and IM-MS, to characterize precisely protein/ligand interaction and to analyze complex mixtures of proteins. Understanding of supramolecular MS instruments and instrumental and methodological optimizations were allowed the development of MS and IM-MS to characterize very high mass supramolecular assembly. Thus, we were able to follow by kinetic the formation of protein/ligand interaction as well as associated conformational modifications, showing the interest of IM-MS coupling in pharmaceutical research. Furthermore, this work deals with the study of high mass complexes and assessment of IM-MS to obtain structural information on these complexes. As a consequence, we have pushed away some limits of MS allowing the use of this technique in structural biology.
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https://tel.archives-ouvertes.fr/tel-00832488
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Submitted on : Monday, June 10, 2013 - 5:42:10 PM
Last modification on : Friday, October 23, 2020 - 5:04:44 PM
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  • HAL Id : tel-00832488, version 1

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Stéphanie Bécard. Développements méthodologiques en spectrométrie de masse et en mobilité ionique pour l'étude d'assemblages supramoléculaires en biologie. Autre [cond-mat.other]. Université de Strasbourg, 2012. Français. ⟨NNT : 2012STRAF036⟩. ⟨tel-00832488⟩

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