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Analyse phénotypique comparative des cellules dentaires et osseuses

Abstract : The oral skeleton is particular by the biodiversity of the mineralized tissues by the presence of bones and teeth. The first part of this work concerns the expression patterns of biomineralization effectors (calbindin-D28k, alkaline phosphatase, amelogenin) during amelogenesis using our model continuously growing rodent tooth and several adapted technical procedure to the study of mineralized tissues. The results show that the patterns of these effectors are controled by a singular way in the dental cells with critical phases for tissue formation. These data are supported by results on specific proteins of the extracellular matrix of enamel during dentin and bone formation. The second part of this thesis is based on the finding of high expression levels of the enamel genes in bone by RT qPCR. Amelogenin, ameloblastin and enamelin are traditionally considered specific for dental cells and tissues. We comparatively evaluated the enamel proteins expression in the jaw and the axial skeleton using descriptive immunohistochemistry and in situ hybridization. Our results show that the oral bone tissue is singularised by enamel proteins and RNA expression.
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Submitted on : Wednesday, May 22, 2013 - 3:27:45 PM
Last modification on : Wednesday, December 9, 2020 - 3:09:21 PM
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  • HAL Id : tel-00824884, version 1


Dominique Hotton. Analyse phénotypique comparative des cellules dentaires et osseuses. Physiologie [q-bio.TO]. Université Pierre et Marie Curie - Paris VI, 2011. Français. ⟨NNT : 2011PA066149⟩. ⟨tel-00824884⟩



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