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La périostine, un nouveau biomarqueur des métastases osseuses : développement d’un immunodosage et évaluation préclinique

Abstract : Periostin is a matricellular protein preferentially expressed at sites subjected to mechanical constraints, including the periosteum, and in the stroma associated to several tumor types. We first aimed to evaluate the relevance of periostin as a biomarker of bone metabolism or stromal reaction in bone metastases. We developed the first ELISA for serum periostin in mouse with analytical characteristics (specificity, precision) that are in accordance with regulatory standards. This ELISA allowed us to specify further the involvement of periostin in bone metabolism and breast cancer bone metastases. Our in vitro and in vivo data suggested that periostin is a component of primary ossification rather than a direct index of bone remodeling, unlike conventional bone markers. In breast cancer bone metastases, we also showed that periostin is overexpressed by stromal cells associated with bone metastasis, in agreement with its localization in the stroma of primary tumors. Finally, using bioinformatics analyses of large datasets from various tumors in human, we confirmed the close relationship between periostin and the stromal reaction. Periostin and other co-expressed proteins could therefore constitute a set of biological markers of cancer progression, and/or appear as potential therapeutic targets.
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https://tel.archives-ouvertes.fr/tel-00824383
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Submitted on : Tuesday, May 21, 2013 - 4:12:15 PM
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Sylvain Contié. La périostine, un nouveau biomarqueur des métastases osseuses : développement d’un immunodosage et évaluation préclinique. Médecine humaine et pathologie. Université Claude Bernard - Lyon I, 2010. Français. ⟨NNT : 2010LYO10253⟩. ⟨tel-00824383⟩

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