Skip to Main content Skip to Navigation
Theses

Régulation temporelle de l’abscission, la dernière étape de la division cellulaire : rôle des forces exercées au niveau du pont intercellulaire

Abstract : The last step of cytokinesis, abscission, consists in the severing of the intercellular bridge connecting the two daughter cells after the contraction of the acto-myosin ring. As any other step of cell division, abscission has to be regulated both in time and space in order to take place at the proper time and proper place. During my PhD, I studied the temporal regulation of the abscission by the cell micro-environment, particularly by the traction forces exerted by the cells on the intercellular bridge. I used a combination of approaches to control the daughter cells 2D spatial confinement, to measure the forces exerted by the cells throughout cytokinesis, and to micro-manipulate the intercellular bridge. Counter-intuitively, a tension exerted on the intercellular bridge delayed abscission while a release of tension in the bridge induced abscission. Moreover, the temporal regulation of abscission by the environment of the daughter cells implies the Endosomal Sorting Complex Required for Transport III (ESCRT-III), the main abscission machinery. Finally, preliminary experiments suggest that this mechanism could be important for tissue integrity and morphogenesis.
Document type :
Theses
Complete list of metadatas

https://tel.archives-ouvertes.fr/tel-00819619
Contributor : Abes Star :  Contact
Submitted on : Thursday, May 2, 2013 - 1:08:57 AM
Last modification on : Monday, December 14, 2020 - 9:44:43 AM

File

VD2_JANVORE_JULIE_28092012.pdf
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-00819619, version 1

Citation

Julie Janvore. Régulation temporelle de l’abscission, la dernière étape de la division cellulaire : rôle des forces exercées au niveau du pont intercellulaire. Sciences agricoles. Université Paris Sud - Paris XI, 2012. Français. ⟨NNT : 2012PA112195⟩. ⟨tel-00819619⟩

Share

Metrics

Record views

802

Files downloads

1670