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Phospholipid signalling in the salicylic acid pathway of Arabidopsis thaliana

Abstract : Salicylic acid (SA) has a pivotal role in many plant stress and developmental responses but little is known about the early signalling events triggered by this molecule. Using Arabidopsis thaliana suspension cells, it was shown by in vivo metabolic phospholipid labelling with 33Pi that SA induced rapid and early decrease in a pool of phosphatidylinositol (PI). The decrease was accompanied by an increase in PI 4-phosphate and PI 4,5-bisphosphate contents. These changes could be inhibited by 30 µM wortmannine, but not by 1 μM wortmannine, implying that a type III PI 4-kinase was activated in response to SA. Therefore, a study of wortmannin effects on SA transcriptome was undertaken. Using the Complete Arabidopsis Transcriptome MicroArray (CATMA) chip, 773 SA-regulated genes were identified. Among these, the SA response of 112 was inhibited by 30 µM wortmannine, but not by 1 µM wortmannin. In parallel, it was discovered that phospholipase D (PLD) derived phosphatidic acid was important for the early SA-regulated gene expression. Microarray experiment aimed to identify genes regulated by PLD in response to SA revealed that out of 1327 genes regulated by SA, 97 genes were positively and 117 genes were negatively regulated by PLD. The wortmannin-sensitive pathway and PLD pathway regulons share an important overlap implying that the two enzyme activities act synergistically in the same signalling pathway in response to SA.
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  • HAL Id : tel-00808969, version 1

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Ondrej Krinke. Phospholipid signalling in the salicylic acid pathway of Arabidopsis thaliana. Biochemistry, Molecular Biology. Université Pierre et Marie Curie - Paris VI, 2007. English. ⟨NNT : 2007PA066228⟩. ⟨tel-00808969⟩

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