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Contribution à la formulation et à l'évaluation de liposomes d'ATP

Abstract : ATP liposome incorporating hepatic ligands may contribute to improve the energetic status of the liver graft. In a first phase of development, it has been emphasized the great need of stabilizing the liposome (i) and of validating a cellular model with an altered energetic status in order to test the formulations of interest. To provide a stable liposomal preparation, different strategies have been carried out to freeze-dry liposome with or without ATP. Sucrose and trehalose better stabilize the liposome preparation during the freeze-drying process. Nevertheless, in all conditions, a significant ATP leakage has been observed. An inhibitor of the cartinine palmitoyl transferase (i.e. Etomoxir) has shown great interest to modulate the energetic level in HepG2 cells by varying Etomoxir concentration and culture temperature. In conclusion, this study contributes to a better understanding of the critical factors related to liposome freeze-drying and describes hepatic cell models with altered energetic status that may have great interest to test specific agent or innovating formulations.
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Véronique Vincourt-Vitse. Contribution à la formulation et à l'évaluation de liposomes d'ATP. Médecine humaine et pathologie. Université René Descartes - Paris V, 2012. Français. ⟨NNT : 2012PA05P651⟩. ⟨tel-00806758⟩

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