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Évolution du génome des spartines polyploïdes envahissant les marais salés : apport des nouvelles techniques de séquençage haut-débit

Abstract : Spartina species play an important ecological role on salt marshes. They represent an excellent system to study the ecological consequences of hybrid and polyploid speciation in biological invasion contexts. In this study, we examined the effects of hybridization between the hexaploid American-native species Spartina alterniflora and the European species S. maritima, that gave rise to two F1 hybrids (S. x townsendii in England et S. x neyrautii in France) and the new invasive allododecaploid species (S. anglica). Next-generation sequencing technologies offer new perspectives to explore these previously poorly known genomes. The assembly of a reference transcriptome (from 454 Roche pyrosequencing) allowed annotation of 16,753 genes in hexaploid Spartina and identification of ecologically and evolutionary important genes. Expression levels of a subset of these genes were analyzed by quantitative PCR in Spartina natural populations. The results indicate intrapopulation homogenous expression but extreme variability between species. The European S. maritima beneficiated from genomic resource development through a BAC library and one pyrosequencing run. Our analyses estimated the relative proportions of repetitive sequences as about 30% and have identified the main transposable element families Data generated were also compared to closely related sequenced species and provided the first insights into the evolution of Spartina genomes in the Poaceae family.
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https://tel.archives-ouvertes.fr/tel-00795861
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Submitted on : Friday, March 1, 2013 - 9:07:27 AM
Last modification on : Thursday, January 14, 2021 - 11:45:00 AM
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Julie Ferreira de Carvalho. Évolution du génome des spartines polyploïdes envahissant les marais salés : apport des nouvelles techniques de séquençage haut-débit. Sciences agricoles. Université Rennes 1, 2013. Français. ⟨NNT : 2013REN1S002⟩. ⟨tel-00795861⟩

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