Identification des moisissures et de leurs métabolites secondaires colonisant des supports papiers : évaluation de la toxicité sur des cellules épithéliales respiratoires in vitro

Abstract : Introduction: Little study has been carried out on the presence of fungi and their mycotoxins in complex paper matrices. Our work has focused on finding mycotoxins on heritage paper altered by the foxing and wallpapers from moldy homes whose residents have been diagnosed with symptoms of allergies and sick building syndrome. Objectives: To identify the fungal species of these two types of paper and to determine the production of fungal metabolites. Materials and methods: The foxing has been characterized using multi-disciplinary technics (physical, biological, bioanalytical, cytotoxicity assays). The fungal metabolites in the hydro-organic extracts of these papers were sought and identified by mass spectrometry to assess whether they could be related to fungal species detected by microbiology. The toxicological risk of wallpaper extracts was then evaluated on model in vitro cells. Results: For the foxing, we could exclude a metal origine and we showed that the microorganisms present are mainly fungal species. For wallpapers, fungal metabolites were found. By seeding individual fungal species on wallpaper and using MS2, we were able to link the compounds of m/z 401 and 487 to S. chartarum. Cytotoxicity tests showed a significant increase in cytotoxicity of A549 cells with moldy wallpaper in comparison with the control wallpaper. A549 cells showed an overexpression of TNF-α after contact with these wallpapers as well as a significant overexpression of IL-8 and CYP 1A1.Discussion-Conclusion: We detected no mycotoxin in foxing, thus excluding possible links between inhalation of mycotoxins from old manuscripts and respiratory symptoms. For people in their homes and respiratory symptoms, it is difficult to draw any relationships to a given fungal species or a metabolite although S. chartarum has been implicated. These initial results should be confirmed by further study
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Sarah Boudih. Identification des moisissures et de leurs métabolites secondaires colonisant des supports papiers : évaluation de la toxicité sur des cellules épithéliales respiratoires in vitro. Sciences agricoles. Université Paris-Est, 2011. Français. ⟨NNT : 2011PEST0002⟩. ⟨tel-00781807⟩

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